Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3467158..3467992 Replicon chromosome
Accession NZ_CP043295
Organism Escherichia coli strain WCHEC040047

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2T3TJB4
Locus tag FYK54_RS16595 Protein ID WP_000854811.1
Coordinates 3467158..3467535 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2T3TJJ3
Locus tag FYK54_RS16600 Protein ID WP_001285590.1
Coordinates 3467624..3467992 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK54_RS16575 (3464956) 3464956..3466176 + 1221 WP_000794251.1 capsular biosynthesis protein -
FYK54_RS16580 (3466202) 3466202..3466432 - 231 Protein_3234 hypothetical protein -
FYK54_RS16585 (3466538) 3466538..3466714 - 177 WP_000839288.1 DUF957 domain-containing protein -
FYK54_RS16590 (3466731) 3466731..3467161 - 431 Protein_3236 DUF5983 family protein -
FYK54_RS16595 (3467158) 3467158..3467535 - 378 WP_000854811.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
FYK54_RS16600 (3467624) 3467624..3467992 - 369 WP_001285590.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
FYK54_RS16605 (3468066) 3468066..3468287 - 222 WP_000692300.1 DUF987 domain-containing protein -
FYK54_RS16610 (3468356) 3468356..3468832 - 477 WP_001186725.1 RadC family protein -
FYK54_RS16615 (3468848) 3468848..3469333 - 486 WP_000214310.1 antirestriction protein -
FYK54_RS16620 (3469425) 3469425..3470243 - 819 WP_001234593.1 DUF932 domain-containing protein -
FYK54_RS16625 (3470361) 3470361..3470532 - 172 Protein_3243 DUF905 family protein -
FYK54_RS16630 (3470633) 3470633..3471088 - 456 WP_000581502.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14041.14 Da        Isoelectric Point: 7.8839

>T138278 WP_000854811.1 NZ_CP043295:c3467535-3467158 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Download         Length: 378 bp

>T138278 NZ_CP057506:c312037-311720 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAGGGATTTAGTAAACGCCGTCCGTTACTTTTACCTGACGATGAGTTTCGTGCCTTTCA
AGAGGCGCTTATTGAAAACCCTGAGGCAGGGGATACTATCGCTGGTACGGGGGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGTAAGCGCAGCGGTATTCGGGTTATCTATTACAACGTGACGCGTAAAGGTCGAATCTATCTGGCTTTGCTC
TATCCTAAGAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCGATATGCTGATTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13608.50 Da        Isoelectric Point: 6.6242

>AT138278 WP_001285590.1 NZ_CP043295:c3467992-3467624 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLYSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT138278 NZ_CP057506:c311667-311371 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTACGTATAGAGAAAGGTGAAGAAACACCTAA
ACCTGAACATGTACATACTTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCGGTTGGTGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAACCCACTGGAGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAACCCATTTATAATTAATCAATTAAAAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2T3TJB4

Antitoxin

Source ID Structure
AlphaFold DB A0A2T3TJJ3

References