Detailed information of TA system TA138273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2048008..2048603
• Replicon: chromosome
• Accession: NZ_CP043295
• Organism: Escherichia coli strain WCHEC040047

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: FYK54_RS09900
• Protein ID: WP_000239581.1
• Coordinates: 2048008..2048358 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: FYK54_RS09905
• Protein ID: WP_001223213.1
• Coordinates: 2048352..2048603 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK54_RS09880 (2043462)	2043462..2044484	-	1023	WP_001361374.1	ABC transporter permease	-
FYK54_RS09885 (2044498)	2044498..2046000	-	1503	WP_001522399.1	sugar ABC transporter ATP-binding protein	-
FYK54_RS09890 (2046133)	2046133..2047089	-	957	WP_001522398.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
FYK54_RS09895 (2047399)	2047399..2047929	+	531	WP_000055075.1	inorganic diphosphatase	-
FYK54_RS09900 (2048008)	2048008..2048358	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
FYK54_RS09905 (2048352)	2048352..2048603	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
FYK54_RS09910 (2048815)	2048815..2049156	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
FYK54_RS09915 (2049159)	2049159..2052938	-	3780	WP_001522396.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T138273 WP_000239581.1 NZ_CP043295:c2048358-2048008 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T138273 NZ_CP057505:c3389671-3389273 [Escherichia fergusonii]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGACAAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACA
AATGACGAAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT138273 WP_001223213.1 NZ_CP043295:c2048603-2048352 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT138273 NZ_CP057505:c3389967-3389674 [Escherichia fergusonii]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATAGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCTATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7