Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1313943..1314561 Replicon chromosome
Accession NZ_CP043295
Organism Escherichia coli strain WCHEC040047

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FYK54_RS06410 Protein ID WP_001291435.1
Coordinates 1314343..1314561 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FYK54_RS06405 Protein ID WP_000344800.1
Coordinates 1313943..1314317 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK54_RS06395 (1309032) 1309032..1310225 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FYK54_RS06400 (1310248) 1310248..1313397 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
FYK54_RS06405 (1313943) 1313943..1314317 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FYK54_RS06410 (1314343) 1314343..1314561 + 219 WP_001291435.1 HHA domain-containing protein Toxin
FYK54_RS06415 (1314733) 1314733..1315284 + 552 WP_001522034.1 maltose O-acetyltransferase -
FYK54_RS06420 (1315400) 1315400..1315870 + 471 WP_001490888.1 YlaC family protein -
FYK54_RS06425 (1316034) 1316034..1317584 + 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FYK54_RS06430 (1317626) 1317626..1317979 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FYK54_RS06440 (1318358) 1318358..1318669 + 312 WP_000409911.1 MGMT family protein -
FYK54_RS06445 (1318700) 1318700..1319272 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T138268 WP_001291435.1 NZ_CP043295:1314343-1314561 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T138268 NZ_CP057504:c4218065-4217913 [Escherichia fergusonii]
ATGCCGCAGAAATATAGATTACTTTCTTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGAGAGTTATGAGCTGGCGGCGTTTTTAGCCTGCAAATTGAAAGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT138268 WP_000344800.1 NZ_CP043295:1313943-1314317 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT138268 NZ_CP043295:1313943-1314317 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References