Detailed information of TA system TA138267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 847105..847810
• Replicon: chromosome
• Accession: NZ_CP043295
• Organism: Escherichia coli strain WCHEC040047

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: FYK54_RS04200
• Protein ID: WP_000539521.1
• Coordinates: 847105..847491 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FYK54_RS04205
• Protein ID: WP_001280945.1
• Coordinates: 847481..847810 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK54_RS04180 (843109)	843109..843735	+	627	WP_020233670.1	glutathione S-transferase GstB	-
FYK54_RS04185 (843732)	843732..844847	-	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
FYK54_RS04190 (844958)	844958..845341	-	384	WP_000497137.1	biofilm formation regulator BssR	-
FYK54_RS04195 (845554)	845554..846879	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
FYK54_RS04200 (847105)	847105..847491	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FYK54_RS04205 (847481)	847481..847810	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
FYK54_RS04210 (847880)	847880..849208	-	1329	WP_000086873.1	GGDEF domain-containing protein	-
FYK54_RS04215 (849216)	849216..851564	-	2349	WP_021523051.1	EAL domain-containing protein	-
FYK54_RS04220 (851742)	851742..852653	-	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T138267 WP_000539521.1 NZ_CP043295:847105-847491 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T138267 NZ_CP057504:c4189215-4189108 [Escherichia fergusonii]
ATGACGCTCGCAGAACTGGGCATGGACTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCAAGTCTGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT138267 WP_001280945.1 NZ_CP043295:847481-847810 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT138267 NZ_CP043295:847481-847810 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6