Detailed information of TA system TA138265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 169455..170093
• Replicon: chromosome
• Accession: NZ_CP043295
• Organism: Escherichia coli strain WCHEC040047

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: FYK54_RS00805
• Protein ID: WP_001306887.1
• Coordinates: 169917..170093 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FYK54_RS00800
• Protein ID: WP_001270286.1
• Coordinates: 169455..169871 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK54_RS00780 (164607)	164607..165548	-	942	WP_001251315.1	ABC transporter permease	-
FYK54_RS00785 (165549)	165549..166562	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
FYK54_RS00790 (166580)	166580..167725	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
FYK54_RS00795 (167970)	167970..169376	-	1407	WP_241418131.1	PLP-dependent aminotransferase family protein	-
FYK54_RS00800 (169455)	169455..169871	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FYK54_RS00805 (169917)	169917..170093	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FYK54_RS00810 (170315)	170315..170545	+	231	WP_000494241.1	YncJ family protein	-
FYK54_RS00815 (170637)	170637..172598	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FYK54_RS00820 (172671)	172671..173207	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
FYK54_RS00825 (173299)	173299..174471	+	1173	WP_001523225.1	BenE family transporter YdcO	-
FYK54_RS00830 (174579)	174579..175037	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	174579..175037	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T138265 WP_001306887.1 NZ_CP043295:c170093-169917 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T138265 NZ_CP057504:c3508294-3507887 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCCCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACCTGTGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT138265 WP_001270286.1 NZ_CP043295:c169871-169455 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT138265 NZ_CP057504:c3508541-3508275 [Escherichia fergusonii]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGTGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76