Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-doc/Doc-Phd |
| Location | 62656..63257 | Replicon | plasmid p1_025985 |
| Accession | NZ_CP043288 | ||
| Organism | Escherichia coli strain WCHEC025985 | ||
Toxin (Protein)
| Gene name | doc | Uniprot ID | U9YA20 |
| Locus tag | FYK42_RS25280 | Protein ID | WP_001216045.1 |
| Coordinates | 62656..63036 (-) | Length | 127 a.a. |
Antitoxin (Protein)
| Gene name | phd | Uniprot ID | U9YQH9 |
| Locus tag | FYK42_RS25285 | Protein ID | WP_001190712.1 |
| Coordinates | 63036..63257 (-) | Length | 74 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FYK42_RS25255 (FYK42_25640) | 58096..59580 | - | 1485 | WP_000124150.1 | terminase | - |
| FYK42_RS25260 (FYK42_25645) | 59580..60773 | - | 1194 | WP_106489616.1 | terminase | - |
| FYK42_RS25265 (FYK42_25650) | 60860..61312 | - | 453 | WP_001326849.1 | late promoter-activating protein | - |
| FYK42_RS25270 (FYK42_25655) | 61401..62444 | - | 1044 | WP_241408131.1 | DUF968 domain-containing protein | - |
| FYK42_RS25275 (FYK42_25660) | 62472..62651 | - | 180 | WP_000113019.1 | hypothetical protein | - |
| FYK42_RS25280 (FYK42_25665) | 62656..63036 | - | 381 | WP_001216045.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin |
| FYK42_RS25285 (FYK42_25670) | 63036..63257 | - | 222 | WP_001190712.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| FYK42_RS25290 (FYK42_25675) | 63330..63719 | - | 390 | WP_000506726.1 | S24 family peptidase | - |
| FYK42_RS25295 (FYK42_25680) | 63843..64094 | - | 252 | WP_032153798.1 | DNA polymerase III subunit theta | - |
| FYK42_RS25300 (FYK42_25685) | 64097..64363 | - | 267 | WP_000903204.1 | hypothetical protein | - |
| FYK42_RS25305 (FYK42_25690) | 64409..65182 | - | 774 | WP_223677252.1 | hypothetical protein | - |
| FYK42_RS25310 (FYK42_25695) | 65164..65538 | - | 375 | WP_032353387.1 | hypothetical protein | - |
| FYK42_RS25315 (FYK42_25700) | 65545..65838 | - | 294 | WP_000269001.1 | hypothetical protein | - |
| FYK42_RS25320 (FYK42_25705) | 66017..66250 | - | 234 | WP_000517421.1 | hypothetical protein | - |
| FYK42_RS25325 (FYK42_25715) | 66333..67019 | - | 687 | WP_234287463.1 | DUF550 domain-containing protein | - |
| FYK42_RS25330 | 66993..67118 | - | 126 | Protein_69 | DUF550 domain-containing protein | - |
| FYK42_RS25335 (FYK42_25720) | 67115..67324 | - | 210 | WP_153061224.1 | hypothetical protein | - |
| FYK42_RS25340 (FYK42_25725) | 67326..67721 | - | 396 | WP_241408132.1 | hypothetical protein | - |
| FYK42_RS25345 (FYK42_25735) | 68055..68233 | - | 179 | Protein_72 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..86177 | 86177 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 127 a.a. Molecular weight: 13588.29 Da Isoelectric Point: 5.1514
>T138258 WP_001216045.1 NZ_CP043288:c63036-62656 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
Download Length: 381 bp
>T138258 NZ_CP043288:c63036-62656 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG
Antitoxin
Download Length: 74 a.a. Molecular weight: 8133.10 Da Isoelectric Point: 4.7708
>AT138258 WP_001190712.1 NZ_CP043288:c63257-63036 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
Download Length: 222 bp
>AT138258 NZ_CP057483:4041387-4041665 [Citrobacter sp. RHB25-C09]
ATGAATAAGCTTACTCAATACGATCCCGCCGCTGCGCTGGTCGATGACGACGAAATTGCTGTATTTATGGCAGATGCCCT
GGAAACGGGTGATGCGGCTTACATAGCAAAAGCGCTCGGCGTGGTGGCTCGCGCAAAAGGCATGGCAAACATTGCGGCTG
AAACCGGCTTATCACGCGAACAGCTTTATCGTTCATTCAGTGACAAAGGCAACCCCACGCTTAAAACCACGCTGGCGGTG
ATGAAGGCGCTGGGTATTGGATTAACTGTTAAAACATGA
ATGAATAAGCTTACTCAATACGATCCCGCCGCTGCGCTGGTCGATGACGACGAAATTGCTGTATTTATGGCAGATGCCCT
GGAAACGGGTGATGCGGCTTACATAGCAAAAGCGCTCGGCGTGGTGGCTCGCGCAAAAGGCATGGCAAACATTGCGGCTG
AAACCGGCTTATCACGCGAACAGCTTTATCGTTCATTCAGTGACAAAGGCAACCCCACGCTTAAAACCACGCTGGCGGTG
ATGAAGGCGCTGGGTATTGGATTAACTGTTAAAACATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CLP7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CJB6 |