Detailed information of TA system TA138254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 73155..73780
• Replicon: plasmid pCTXM55_025985
• Accession: NZ_CP043286
• Organism: Escherichia coli strain WCHEC025985

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FYK42_RS24430
• Protein ID: WP_000911313.1
• Coordinates: 73382..73780 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: FYK42_RS24425
• Protein ID: WP_000450520.1
• Coordinates: 73155..73382 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK42_RS24425 (73155)	73155..73382	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FYK42_RS24430 (73382)	73382..73780	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
FYK42_RS24435 (73789)	73789..75579	-	1791	Protein_85	type IV conjugative transfer system coupling protein TraD	-
FYK42_RS24440 (75575)	75575..76816	-	1242	Protein_86	conjugal transfer mating-pair stabilization protein TraG	-
FYK42_RS24445 (76813)	76813..78186	-	1374	WP_001517790.1	conjugal transfer pilus assembly protein TraH	-
FYK42_RS24450 (78186)	78186..78566	-	381	WP_001389370.1	F-type conjugal transfer protein TrbF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / cmlA1 / ARR-3 / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / ant(3'')-Ia / lnu(F) / sitABCD	iutA / iucD / iucC / iucB / iucB / iucA	1..111058	111058

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T138254 WP_000911313.1 NZ_CP043286:73382-73780 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T138254 NZ_CP043286:73382-73780 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT138254 WP_000450520.1 NZ_CP043286:73155-73382 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT138254 NZ_CP043286:73155-73382 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2