Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21104..21747 Replicon plasmid pCTXM55_025985
Accession NZ_CP043286
Organism Escherichia coli strain WCHEC025985

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag FYK42_RS24140 Protein ID WP_001044768.1
Coordinates 21331..21747 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag FYK42_RS24135 Protein ID WP_001261287.1
Coordinates 21104..21334 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK42_RS24115 (16264) 16264..17352 + 1089 WP_000952217.1 transcriptional repressor PifC -
FYK42_RS24120 (17354) 17354..19579 + 2226 WP_000698737.1 P-loop NTPase fold protein -
FYK42_RS24125 (19629) 19629..20528 - 900 WP_000963206.1 nucleotide-binding protein -
FYK42_RS24130 (20518) 20518..20808 - 291 WP_000111771.1 hypothetical protein -
FYK42_RS24135 (21104) 21104..21334 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FYK42_RS24140 (21331) 21331..21747 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
FYK42_RS24145 (21909) 21909..24047 - 2139 WP_000350638.1 AAA family ATPase -
FYK42_RS24150 (24401) 24401..24658 + 258 WP_000343085.1 hypothetical protein -
FYK42_RS24155 (24658) 24658..25248 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-55 / cmlA1 / ARR-3 / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / ant(3'')-Ia / lnu(F) / sitABCD iutA / iucD / iucC / iucB / iucB / iucA 1..111058 111058


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T138249 WP_001044768.1 NZ_CP043286:21331-21747 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T138249 NZ_CP043286:21331-21747 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT138249 WP_001261287.1 NZ_CP043286:21104-21334 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT138249 NZ_CP043286:21104-21334 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References