Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4291216..4291713 Replicon chromosome
Accession NZ_CP043284
Organism Escherichia coli strain WCHEC025985

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FYK42_RS20995 Protein ID WP_001173343.1
Coordinates 4291216..4291389 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag FYK42_RS21000 Protein ID WP_001425728.1
Coordinates 4291417..4291713 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK42_RS20985 (4287886) 4287886..4289343 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
FYK42_RS20990 (4289580) 4289580..4291097 + 1518 WP_001295074.1 lysine--tRNA ligase -
FYK42_RS20995 (4291216) 4291216..4291389 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FYK42_RS21000 (4291417) 4291417..4291713 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FYK42_RS21005 (4291940) 4291940..4292212 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
FYK42_RS21010 (4292224) 4292224..4292454 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
FYK42_RS21015 (4292635) 4292635..4294266 + 1632 WP_001216487.1 sensor histidine kinase -
FYK42_RS21020 (4294263) 4294263..4294982 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T138248 WP_001173343.1 NZ_CP043284:c4291389-4291216 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T138248 NZ_CP057481:3831000-3831341 [Escherichia fergusonii]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCCCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAACTGTCGGCGCGTAAACAGAAGCAGGTGCAGGAGTTTATTGGGGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT138248 WP_001425728.1 NZ_CP043284:c4291713-4291417 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT138248 NZ_CP057481:c3831005-3830929 [Escherichia fergusonii]
AGTCATAACTGCTATTCTCCTGGAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References