Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3511438..3512056 Replicon chromosome
Accession NZ_CP043284
Organism Escherichia coli strain WCHEC025985

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FYK42_RS17145 Protein ID WP_001291435.1
Coordinates 3511838..3512056 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FYK42_RS17140 Protein ID WP_000344800.1
Coordinates 3511438..3511812 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK42_RS17130 (3506527) 3506527..3507720 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FYK42_RS17135 (3507743) 3507743..3510892 + 3150 WP_241408057.1 efflux RND transporter permease AcrB -
FYK42_RS17140 (3511438) 3511438..3511812 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FYK42_RS17145 (3511838) 3511838..3512056 + 219 WP_001291435.1 HHA domain-containing protein Toxin
FYK42_RS17150 (3512228) 3512228..3512779 + 552 WP_000102568.1 maltose O-acetyltransferase -
FYK42_RS17155 (3512895) 3512895..3513365 + 471 WP_000136192.1 YlaC family protein -
FYK42_RS17160 (3513529) 3513529..3515079 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FYK42_RS17165 (3515121) 3515121..3515474 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FYK42_RS17175 (3515853) 3515853..3516164 + 312 WP_000409911.1 MGMT family protein -
FYK42_RS17180 (3516195) 3516195..3516767 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T138247 WP_001291435.1 NZ_CP043284:3511838-3512056 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T138247 NZ_CP043284:3511838-3512056 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT138247 WP_000344800.1 NZ_CP043284:3511438-3511812 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT138247 NZ_CP057481:c3822808-3822506 [Escherichia fergusonii]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAATAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References