Detailed information of TA system TA138236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1380046..1380671
• Replicon: chromosome
• Accession: NZ_CP043284
• Organism: Escherichia coli strain WCHEC025985

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FYK42_RS06765
• Protein ID: WP_000911330.1
• Coordinates: 1380273..1380671 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FYK42_RS06760
• Protein ID: WP_000450524.1
• Coordinates: 1380046..1380273 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK42_RS06735 (1375849)	1375849..1376319	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
FYK42_RS06740 (1376319)	1376319..1376891	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FYK42_RS06745 (1377037)	1377037..1377915	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FYK42_RS06750 (1377932)	1377932..1378966	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FYK42_RS06755 (1379179)	1379179..1379892	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FYK42_RS06760 (1380046)	1380046..1380273	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FYK42_RS06765 (1380273)	1380273..1380671	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
FYK42_RS06770 (1380818)	1380818..1381681	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
FYK42_RS06775 (1381696)	1381696..1383711	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
FYK42_RS06780 (1383785)	1383785..1384483	+	699	WP_000679823.1	esterase	-
FYK42_RS06785 (1384593)	1384593..1384793	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T138236 WP_000911330.1 NZ_CP043284:1380273-1380671 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T138236 NZ_CP057481:775398-775712 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT138236 WP_000450524.1 NZ_CP043284:1380046-1380273 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT138236 NZ_CP043284:1380046-1380273 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33