Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 949603..950257 Replicon chromosome
Accession NZ_CP043284
Organism Escherichia coli strain WCHEC025985

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FYK42_RS04695 Protein ID WP_000244777.1
Coordinates 949850..950257 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FYK42_RS04690 Protein ID WP_000354046.1
Coordinates 949603..949869 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK42_RS04665 (944891) 944891..945634 + 744 Protein_918 SDR family oxidoreductase -
FYK42_RS04670 (945691) 945691..947124 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
FYK42_RS04675 (947169) 947169..947480 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
FYK42_RS04680 (947644) 947644..948303 + 660 WP_000250274.1 hemolysin III family protein -
FYK42_RS04685 (948380) 948380..949360 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FYK42_RS04690 (949603) 949603..949869 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FYK42_RS04695 (949850) 949850..950257 + 408 WP_000244777.1 protein YgfX Toxin
FYK42_RS04700 (950297) 950297..950818 - 522 WP_001055874.1 flavodoxin FldB -
FYK42_RS04705 (950930) 950930..951826 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FYK42_RS04710 (951851) 951851..952561 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FYK42_RS04715 (952567) 952567..954300 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T138233 WP_000244777.1 NZ_CP043284:949850-950257 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T138233 NZ_CP057481:554800-555090 [Escherichia fergusonii]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACAAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCTGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT138233 WP_000354046.1 NZ_CP043284:949603-949869 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT138233 NZ_CP043284:949603-949869 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References