Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 318255..319055 Replicon chromosome
Accession NZ_CP043284
Organism Escherichia coli strain WCHEC025985

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag FYK42_RS01490 Protein ID WP_000342449.1
Coordinates 318528..319055 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag FYK42_RS01485 Protein ID WP_001277108.1
Coordinates 318255..318521 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK42_RS01465 (313912) 313912..314580 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
FYK42_RS01470 (314573) 314573..315631 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
FYK42_RS01475 (315876) 315876..316730 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
FYK42_RS01480 (317002) 317002..318105 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
FYK42_RS01485 (318255) 318255..318521 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
FYK42_RS01490 (318528) 318528..319055 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
FYK42_RS01495 (319052) 319052..319435 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
FYK42_RS01500 (319859) 319859..320968 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
FYK42_RS01505 (321016) 321016..321942 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
FYK42_RS01510 (321939) 321939..323216 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
FYK42_RS01515 (323213) 323213..323980 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T138230 WP_000342449.1 NZ_CP043284:318528-319055 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T138230 NZ_CP057463:4086561-4086863 [Escherichia fergusonii]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCACTCAACGCCACATAGAAA
GATTCCTGCTGAAATCCATACCACATTATCAAGAAAATTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGCAGAATTGTATTTAGATCCACACAACTACTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT138230 WP_001277108.1 NZ_CP043284:318255-318521 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT138230 NZ_CP043284:318255-318521 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References