Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4305499..4305996 Replicon chromosome
Accession NZ_CP043279
Organism Escherichia coli strain WCHEC025157

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FYK41_RS20605 Protein ID WP_001173343.1
Coordinates 4305499..4305672 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FYK41_RS20610 Protein ID WP_000398619.1
Coordinates 4305700..4305996 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK41_RS20595 (4302169) 4302169..4303626 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
FYK41_RS20600 (4303863) 4303863..4305380 + 1518 WP_001295074.1 lysine--tRNA ligase -
FYK41_RS20605 (4305499) 4305499..4305672 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FYK41_RS20610 (4305700) 4305700..4305996 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FYK41_RS20615 (4306223) 4306223..4306495 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
FYK41_RS20620 (4306507) 4306507..4306737 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
FYK41_RS20625 (4306918) 4306918..4308549 + 1632 WP_001216477.1 sensor histidine kinase -
FYK41_RS20630 (4308546) 4308546..4309265 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T138218 WP_001173343.1 NZ_CP043279:c4305672-4305499 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T138218 NZ_CP043279:c4305672-4305499 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT138218 WP_000398619.1 NZ_CP043279:c4305996-4305700 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT138218 NZ_CP043279:c4305996-4305700 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References