Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4086828..4087404 Replicon chromosome
Accession NZ_CP043279
Organism Escherichia coli strain WCHEC025157

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag FYK41_RS19465 Protein ID WP_001297643.1
Coordinates 4087117..4087404 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag FYK41_RS19460 Protein ID WP_000063160.1
Coordinates 4086828..4087130 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK41_RS19445 (4083424) 4083424..4085574 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
FYK41_RS19450 (4085624) 4085624..4085827 + 204 WP_000467859.1 YbdD/YjiX family protein -
FYK41_RS19455 (4085838) 4085838..4086794 + 957 WP_001297640.1 GTPase -
FYK41_RS19460 (4086828) 4086828..4087130 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
FYK41_RS19465 (4087117) 4087117..4087404 - 288 WP_001297643.1 BrnT family toxin Toxin
FYK41_RS24670 (4087603) 4087603..4088299 - 697 Protein_3829 IS1 family transposase -
FYK41_RS19480 (4088614) 4088614..4089846 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
FYK41_RS19485 (4089887) 4089887..4091167 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4087603..4087812 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T138216 WP_001297643.1 NZ_CP043279:c4087404-4087117 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T138216 NZ_CP057463:c520535-520257 [Escherichia fergusonii]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGTGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATCTCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTTGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT138216 WP_000063160.1 NZ_CP043279:c4087130-4086828 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT138216 NZ_CP057463:c520257-519973 [Escherichia fergusonii]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTTACCGGAAAAGCGGCATTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTAGATGTGTCGCGACTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References