Detailed information of TA system TA138212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2460640..2461278
• Replicon: chromosome
• Accession: NZ_CP043279
• Organism: Escherichia coli strain WCHEC025157

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: FYK41_RS11690
• Protein ID: WP_001447010.1
• Coordinates: 2461102..2461278 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FYK41_RS11685
• Protein ID: WP_001270286.1
• Coordinates: 2460640..2461056 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK41_RS11665 (2455792)	2455792..2456733	-	942	WP_001251304.1	ABC transporter permease	-
FYK41_RS11670 (2456734)	2456734..2457747	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
FYK41_RS11675 (2457765)	2457765..2458910	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
FYK41_RS11680 (2459155)	2459155..2460561	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
FYK41_RS11685 (2460640)	2460640..2461056	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FYK41_RS11690 (2461102)	2461102..2461278	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FYK41_RS11695 (2461500)	2461500..2461730	+	231	WP_000494244.1	YncJ family protein	-
FYK41_RS11700 (2461822)	2461822..2463783	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FYK41_RS11705 (2463856)	2463856..2464392	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
FYK41_RS11710 (2464484)	2464484..2465659	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T138212 WP_001447010.1 NZ_CP043279:c2461278-2461102 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T138212 NZ_CP043279:c2461278-2461102 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT138212 WP_001270286.1 NZ_CP043279:c2461056-2460640 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT138212 NZ_CP043279:c2461056-2460640 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8