Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 929345..929999 Replicon chromosome
Accession NZ_CP043279
Organism Escherichia coli strain WCHEC025157

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FYK41_RS04555 Protein ID WP_000244777.1
Coordinates 929592..929999 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FYK41_RS04550 Protein ID WP_000354046.1
Coordinates 929345..929611 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK41_RS04525 (924514) 924514..925257 + 744 WP_000951964.1 SDR family oxidoreductase -
FYK41_RS04530 (925314) 925314..926747 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
FYK41_RS04535 (926792) 926792..927103 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
FYK41_RS04540 (927267) 927267..927926 + 660 WP_000250274.1 hemolysin III family protein -
FYK41_RS04545 (928122) 928122..929102 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FYK41_RS04550 (929345) 929345..929611 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FYK41_RS04555 (929592) 929592..929999 + 408 WP_000244777.1 protein YgfX Toxin
FYK41_RS04560 (930039) 930039..930560 - 522 WP_001055874.1 flavodoxin FldB -
FYK41_RS04565 (930672) 930672..931568 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FYK41_RS04570 (931593) 931593..932303 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FYK41_RS04575 (932309) 932309..934042 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T138200 WP_000244777.1 NZ_CP043279:929592-929999 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T138200 NZ_CP057458:c4118830-4118723 [Escherichia fergusonii]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCTGCTCCGGTCATTACTGGCATTCTTGCCAGTCTGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT138200 WP_000354046.1 NZ_CP043279:929345-929611 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT138200 NZ_CP057458:4118888-4118942 [Escherichia fergusonii]
CAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References