Detailed information of TA system TA138194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 50114..50715
• Replicon: plasmid p2_035148
• Accession: NZ_CP043277
• Organism: Escherichia coli strain WCHEC035148

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: FYK53_RS23940
• Protein ID: WP_112031922.1
• Coordinates: 50114..50494 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: FYK53_RS23945
• Protein ID: WP_001190712.1
• Coordinates: 50494..50715 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK53_RS23910 (FYK53_24140)	46199..46543	+	345	WP_001557739.1	hypothetical protein	-
FYK53_RS23915 (FYK53_24145)	46540..47016	+	477	WP_000861173.1	hypothetical protein	-
FYK53_RS23920 (FYK53_24150)	47013..47507	+	495	WP_112031919.1	dUTP diphosphatase	-
FYK53_RS23925 (FYK53_24155)	47522..48223	+	702	WP_089477112.1	DUF2829 domain-containing protein	-
FYK53_RS23930 (FYK53_24160)	48230..48994	+	765	WP_112031920.1	hypothetical protein	-
FYK53_RS23935 (FYK53_24165)	48984..50078	+	1095	WP_112031921.1	hypothetical protein	-
FYK53_RS23940 (FYK53_24170)	50114..50494	-	381	WP_112031922.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
FYK53_RS23945 (FYK53_24175)	50494..50715	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FYK53_RS23950 (FYK53_24180)	50788..51177	-	390	WP_000506731.1	S24 family peptidase	-
FYK53_RS23955 (FYK53_24185)	51276..51527	-	252	WP_112031923.1	DNA polymerase III subunit theta	-
FYK53_RS23960 (FYK53_24195)	51842..52672	-	831	WP_112031924.1	DUF551 domain-containing protein	-
FYK53_RS23965 (FYK53_24200)	52683..52946	-	264	WP_000224218.1	hypothetical protein	-
FYK53_RS23970 (FYK53_24205)	52948..53139	-	192	WP_021517457.1	hypothetical protein	-
FYK53_RS23975 (FYK53_24210)	53312..53758	-	447	WP_112031925.1	ead/Ea22-like family protein	-
FYK53_RS23980 (FYK53_24215)	53755..54441	-	687	WP_080028522.1	ead/Ea22-like family protein	-
FYK53_RS23985 (FYK53_24220)	54428..55117	-	690	WP_112031926.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89248	89248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13637.50 Da
• Isoelectric Point: 5.6342

>T138194 WP_112031922.1 NZ_CP043277:c50494-50114 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRDLYGSNI

Nucleotide

• Length: 381 bp

>T138194 NZ_CP057458:c2061867-2061589 [Escherichia fergusonii]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAAT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAACAAGCTGTCTTGCTGATCTCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTTGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT138194 WP_001190712.1 NZ_CP043277:c50715-50494 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT138194 NZ_CP057458:c2061589-2061305 [Escherichia fergusonii]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTTACCGGAAAAGCGGCATTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTAGATGTGTCGCGACTACGCCGTCTGGTTACGCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6