Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 98720..99245 Replicon plasmid p1_035148
Accession NZ_CP043276
Organism Escherichia coli strain WCHEC035148

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FYK53_RS23575 Protein ID WP_001159871.1
Coordinates 98720..99025 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag FYK53_RS23580 Protein ID WP_000813630.1
Coordinates 99027..99245 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK53_RS23560 (94682) 94682..95848 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
FYK53_RS23565 (96436) 96436..97191 - 756 WP_000852146.1 replication initiation protein RepE -
FYK53_RS23570 (97913) 97913..98719 - 807 WP_000016970.1 site-specific integrase -
FYK53_RS23575 (98720) 98720..99025 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FYK53_RS23580 (99027) 99027..99245 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FYK53_RS23585 (99805) 99805..100035 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
FYK53_RS23590 (100032) 100032..100448 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
FYK53_RS23595 (100523) 100523..102088 + 1566 WP_001128474.1 AAA family ATPase -
FYK53_RS23600 (102073) 102073..103095 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / qnrS1 / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..122305 122305
- flank IS/Tn - - 103349..103726 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T138192 WP_001159871.1 NZ_CP043276:c99025-98720 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T138192 NZ_CP057458:c555535-555326 [Escherichia fergusonii]
ATGCTGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT138192 WP_000813630.1 NZ_CP043276:c99245-99027 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT138192 NZ_CP057458:555527-555584 [Escherichia fergusonii]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References