Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3428545..3429163 Replicon chromosome
Accession NZ_CP043274
Organism Escherichia coli strain WCHEC035148

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FYK53_RS16730 Protein ID WP_001291435.1
Coordinates 3428945..3429163 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FYK53_RS16725 Protein ID WP_000344800.1
Coordinates 3428545..3428919 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK53_RS16715 (3423634) 3423634..3424827 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FYK53_RS16720 (3424850) 3424850..3427999 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
FYK53_RS16725 (3428545) 3428545..3428919 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FYK53_RS16730 (3428945) 3428945..3429163 + 219 WP_001291435.1 HHA domain-containing protein Toxin
FYK53_RS16735 (3429335) 3429335..3429886 + 552 WP_000102568.1 maltose O-acetyltransferase -
FYK53_RS16740 (3430002) 3430002..3430472 + 471 WP_000136192.1 YlaC family protein -
FYK53_RS16745 (3430636) 3430636..3432186 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FYK53_RS16750 (3432228) 3432228..3432581 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FYK53_RS16760 (3432960) 3432960..3433271 + 312 WP_000409911.1 MGMT family protein -
FYK53_RS16765 (3433302) 3433302..3433874 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T138180 WP_001291435.1 NZ_CP043274:3428945-3429163 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T138180 NZ_CP043274:3428945-3429163 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT138180 WP_000344800.1 NZ_CP043274:3428545-3428919 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT138180 NZ_CP043274:3428545-3428919 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References