Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1341730..1342355 Replicon chromosome
Accession NZ_CP043274
Organism Escherichia coli strain WCHEC035148

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag FYK53_RS06530 Protein ID WP_000911330.1
Coordinates 1341957..1342355 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag FYK53_RS06525 Protein ID WP_000450524.1
Coordinates 1341730..1341957 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK53_RS06500 (1337533) 1337533..1338003 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
FYK53_RS06505 (1338003) 1338003..1338575 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
FYK53_RS06510 (1338721) 1338721..1339599 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
FYK53_RS06515 (1339616) 1339616..1340650 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
FYK53_RS06520 (1340863) 1340863..1341576 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
FYK53_RS06525 (1341730) 1341730..1341957 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
FYK53_RS06530 (1341957) 1341957..1342355 + 399 WP_000911330.1 type II toxin-antitoxin system VapC family toxin Toxin
FYK53_RS06535 (1342502) 1342502..1343365 + 864 WP_001267507.1 neutral zinc metallopeptidase -
FYK53_RS06540 (1343380) 1343380..1345395 + 2016 WP_000829323.1 tRNA cytosine(34) acetyltransferase TmcA -
FYK53_RS06545 (1345469) 1345469..1346167 + 699 WP_000679823.1 esterase -
FYK53_RS06550 (1346277) 1346277..1346477 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14894.25 Da        Isoelectric Point: 9.2216

>T138170 WP_000911330.1 NZ_CP043274:1341957-1342355 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T138170 NZ_CP057353:4351681-4351968 [Citrobacter freundii]
ATGAGTTATACGGTAAAGTTCAGGGAAGATGCGCTGAAAGAATGGCAAAAATTGGATAAAACCATTCAGCAGCAGTTTGC
CAAAGAACTTAAGAAATGCGGTGAAAATCCGCATGTCCCCTCCGCGAAGCTCAGGGGAATAAAAGATTGCTATAAAATCC
AACTACGCACTTCAAGGTTCCGTCTTGTTTATCAAGTTATTGACGACACCCTGGTCATCGCTGTCGTTGCTGTCGGTAAA
CGCGAGCGTAGTGAAGTTTATAATCTTGCCAGCGAAAGACTACGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT138170 WP_000450524.1 NZ_CP043274:1341730-1341957 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT138170 NZ_CP057353:4351433-4351684 [Citrobacter freundii]
ATGCCACATATCATTTTAAGCGACACCACCGCGAGCGTGAGTGAGCTAAAAAAGAACCCGATGGCGACTGTTAGCGCAGG
GGATGGGTTTCCGGTCGCAATCCTGAACCGTAACCAGCCCGCCTTTTATTGTGTTCCCGCTGAGCTTTACGAAAAAATGC
TTGATGCGCTGGACGATCAGCAACTGGTTAAATTAGTCCATGAACGGAGCCAACAGCCACTGGTGGATGTTGATCTGGAC
AGCTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References