Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 902013..902667 Replicon chromosome
Accession NZ_CP043274
Organism Escherichia coli strain WCHEC035148

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FYK53_RS04445 Protein ID WP_000244777.1
Coordinates 902260..902667 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FYK53_RS04440 Protein ID WP_000354046.1
Coordinates 902013..902279 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK53_RS04415 (897301) 897301..898044 + 744 Protein_870 SDR family oxidoreductase -
FYK53_RS04420 (898101) 898101..899534 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
FYK53_RS04425 (899579) 899579..899890 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
FYK53_RS04430 (900054) 900054..900713 + 660 WP_000250274.1 hemolysin III family protein -
FYK53_RS04435 (900790) 900790..901770 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FYK53_RS04440 (902013) 902013..902279 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FYK53_RS04445 (902260) 902260..902667 + 408 WP_000244777.1 protein YgfX Toxin
FYK53_RS04450 (902707) 902707..903228 - 522 WP_001055874.1 flavodoxin FldB -
FYK53_RS04455 (903340) 903340..904236 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FYK53_RS04460 (904261) 904261..904971 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FYK53_RS04465 (904977) 904977..906710 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T138167 WP_000244777.1 NZ_CP043274:902260-902667 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T138167 NZ_CP043274:902260-902667 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT138167 WP_000354046.1 NZ_CP043274:902013-902279 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT138167 NZ_CP057353:c2328118-2327834 [Citrobacter freundii]
ATGAAAATGGCTAATCATCCCCGTCCGGGGGATATTATTCAGGAAGCGCTGGATGAGTTAAACGTCAGCCTGCGCGAATT
TGCCAGAGCAATGGAGATTGCCCCCTCAACCGCGAGCCGACTGTTAACGGGCAAGGCAGCATTAACCCCGGAAATGGCGA
TCAAACTTTCAGTGGTGATCGGCAGTTCGCCCGAAATGTGGTTGAATCTGCAGAATGCCTGGAGTCTGGCTGAAGCGCAA
AAAAGCGTGGATATCTCACGTCTGCGCAAACTGGTAGCACAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References