Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 295650..296236 Replicon chromosome
Accession NZ_CP043274
Organism Escherichia coli strain WCHEC035148

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag FYK53_RS01340 Protein ID WP_042014162.1
Coordinates 295650..295880 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag FYK53_RS01345 Protein ID WP_000593555.1
Coordinates 295877..296236 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK53_RS01330 (291687) 291687..294422 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
FYK53_RS01335 (294422) 294422..295546 + 1125 WP_001314210.1 ABC transporter permease -
FYK53_RS01340 (295650) 295650..295880 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
FYK53_RS01345 (295877) 295877..296236 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FYK53_RS01350 (296356) 296356..296757 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
FYK53_RS01355 (296763) 296763..297569 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
FYK53_RS01360 (297566) 297566..298330 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
FYK53_RS01365 (298330) 298330..299163 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
FYK53_RS01370 (299160) 299160..300104 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T138163 WP_042014162.1 NZ_CP043274:295650-295880 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T138163 NZ_CP043274:295650-295880 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT138163 WP_000593555.1 NZ_CP043274:295877-296236 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT138163 NZ_CP043274:295877-296236 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References