Detailed information of TA system TA138161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 35659..36140
• Replicon: plasmid p1RM9973
• Accession: NZ_CP043273
• Organism: Escherichia albertii strain RM9973

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FYK17_RS22995
• Protein ID: WP_207610780.1
• Coordinates: 35925..36140 (+)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A3Z6J955
• Locus tag: FYK17_RS22990
• Protein ID: WP_071940833.1
• Coordinates: 35659..35928 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK17_RS22950	30676..31236	+	561	WP_001587438.1	fertility inhibition protein FinO	-
FYK17_RS22955	31372..31584	+	213	WP_001299730.1	ANR family transcriptional regulator	-
FYK17_RS22960	31830..31904	+	75	Protein_36	endonuclease	-
-	32160..32221	-	62	NuclAT_0	-	-
-	32160..32221	-	62	NuclAT_0	-	-
-	32160..32221	-	62	NuclAT_0	-	-
-	32160..32221	-	62	NuclAT_0	-	-
FYK17_RS22965	32208..32414	+	207	WP_207610778.1	Hok/Gef family protein	-
FYK17_RS22970	32698..32955	+	258	WP_000083845.1	replication regulatory protein RepA	-
FYK17_RS22975	33187..33261	+	75	WP_001442103.1	RepA leader peptide Tap	-
FYK17_RS22980	33254..33736	+	483	WP_001523473.1	hypothetical protein	-
FYK17_RS22985	33729..34586	+	858	WP_207610779.1	incFII family plasmid replication initiator RepA	-
FYK17_RS22990	35659..35928	+	270	WP_071940833.1	hypothetical protein	Antitoxin
FYK17_RS22995	35925..36140	+	216	WP_207610780.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FYK17_RS23000	36085..36207	+	123	WP_207610781.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FYK17_RS23005	36314..37477	+	1164	WP_207610782.1	hypothetical protein	-
FYK17_RS23010	37690..38271	-	582	WP_001196199.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..105082	105082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-60)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 7994.16 Da
• Isoelectric Point: 4.9825

>T138161 WP_207610780.1 NZ_CP043273:35925-36140 [Escherichia albertii]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASGLAGQPYKGCNGRVEGTRELVYSPAFCSGL

Nucleotide

• Length: 216 bp

>T138161 NZ_CP043273:35925-36140 [Escherichia albertii]
ATGATGGAGATATTCTGGACCATGCTGGCAAGCCAGGACAGAAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGTGGCCTGGCCGGGCAGCCGTATAAAGGTTGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTTATTCACCCGCATTTTGTTCTGGTTTATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10311.69 Da
• Isoelectric Point: 10.1734

>AT138161 WP_071940833.1 NZ_CP043273:35659-35928 [Escherichia albertii]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLKRGDVLPHDMVESSATAWRD
EMSRKVTGK

Nucleotide

• Length: 270 bp

>AT138161 NZ_CP057331:83338-83631 [Klebsiella oxytoca]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3Z6J955