Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4548523..4549099 Replicon chromosome
Accession NZ_CP043271
Organism Escherichia albertii strain RM9973

Toxin (Protein)


Gene name shpA Uniprot ID A0A0K4FW98
Locus tag FYK17_RS21600 Protein ID WP_044708725.1
Coordinates 4548523..4548810 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0K4FU62
Locus tag FYK17_RS21605 Protein ID WP_054409941.1
Coordinates 4548797..4549099 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK17_RS21585 4544132..4545487 - 1356 WP_054409944.1 restriction endonuclease subunit S -
FYK17_RS21590 4545477..4547096 - 1620 WP_054409942.1 type I restriction-modification system subunit M -
FYK17_RS21595 4547160..4548326 - 1167 WP_059244541.1 restriction endonuclease -
FYK17_RS21600 4548523..4548810 + 288 WP_044708725.1 BrnT family toxin Toxin
FYK17_RS21605 4548797..4549099 + 303 WP_054409941.1 BrnA antitoxin family protein Antitoxin
FYK17_RS21610 4549133..4550089 - 957 WP_054409938.1 GTPase -
FYK17_RS21615 4550100..4550303 - 204 WP_000467859.1 YbdD/YjiX family protein -
FYK17_RS21620 4550353..4552503 - 2151 WP_059221695.1 pyruvate/proton symporter BtsT -
FYK17_RS21625 4553028..4553132 + 105 Protein_4207 Tar ligand binding domain-containing protein -
FYK17_RS21630 4553163..4553294 + 132 WP_024164650.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11155.59 Da        Isoelectric Point: 6.9903

>T138154 WP_044708725.1 NZ_CP043271:4548523-4548810 [Escherichia albertii]
MPMEFEWDANKAKSNQVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T138154 NZ_CP043271:4548523-4548810 [Escherichia albertii]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCAGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTACACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.04 Da        Isoelectric Point: 9.4646

>AT138154 WP_054409941.1 NZ_CP043271:4548797-4549099 [Escherichia albertii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDELWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT138154 NZ_CP043271:4548797-4549099 [Escherichia albertii]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGAGATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCTATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4FW98

Antitoxin

Source ID Structure
AlphaFold DB A0A0K4FU62

References