Detailed information of TA system TA138146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3535920..3536720
• Replicon: chromosome
• Accession: NZ_CP043271
• Organism: Escherichia albertii strain RM9973

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: B1EHN9
• Locus tag: FYK17_RS16955
• Protein ID: WP_000342453.1
• Coordinates: 3535920..3536447 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: B1EHP0
• Locus tag: FYK17_RS16960
• Protein ID: WP_001277106.1
• Coordinates: 3536454..3536720 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK17_RS16930	3531009..3531776	-	768	WP_044709901.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
FYK17_RS16935	3531773..3533050	-	1278	WP_207610263.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-
FYK17_RS16940	3533047..3533973	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
FYK17_RS16945	3534021..3535130	-	1110	WP_207610264.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein	-
FYK17_RS16950	3535540..3535923	+	384	WP_000778774.1	aspartate 1-decarboxylase autocleavage activator PanM	-
FYK17_RS16955	3535920..3536447	-	528	WP_000342453.1	GNAT family N-acetyltransferase	Toxin
FYK17_RS16960	3536454..3536720	-	267	WP_001277106.1	DUF1778 domain-containing protein	Antitoxin
FYK17_RS16965	3536870..3537973	-	1104	WP_010319184.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
FYK17_RS16970	3538496..3538897	+	402	WP_001071334.1	PTS sugar transporter subunit IIA	-
FYK17_RS16975	3538904..3539389	+	486	WP_000029259.1	PTS sugar transporter subunit IIB	-
FYK17_RS16980	3539406..3540152	+	747	WP_000151888.1	PTS sugar transporter subunit IIC	-
FYK17_RS16985	3540145..3540999	+	855	WP_000370583.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19676.58 Da
• Isoelectric Point: 6.9585

>T138146 WP_000342453.1 NZ_CP043271:c3536447-3535920 [Escherichia albertii]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T138146 NZ_CP057313:3872701-3872919 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9942.37 Da
• Isoelectric Point: 10.0863

>AT138146 WP_001277106.1 NZ_CP043271:c3536720-3536454 [Escherichia albertii]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT138146 NZ_CP057313:3872300-3872674 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7Z8E004