Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 42971..43496 Replicon plasmid p1RM9974
Accession NZ_CP043270
Organism Escherichia albertii strain RM9974

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag FYK18_RS25940 Protein ID WP_059242593.1
Coordinates 42971..43276 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FYK18_RS25945 Protein ID WP_000813634.1
Coordinates 43278..43496 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK18_RS25925 (FYK18_26415) 38934..40100 - 1167 WP_059242498.1 plasmid-partitioning protein SopA -
FYK18_RS25930 (FYK18_26420) 40688..41443 - 756 WP_265451253.1 replication initiation protein RepE -
FYK18_RS25935 (FYK18_26425) 42164..42970 - 807 WP_059242594.1 site-specific integrase -
FYK18_RS25940 (FYK18_26430) 42971..43276 - 306 WP_059242593.1 type II toxin-antitoxin system toxin CcdB Toxin
FYK18_RS25945 (FYK18_26435) 43278..43496 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FYK18_RS25950 (FYK18_26445) 44093..44347 + 255 WP_059242592.1 hypothetical protein -
FYK18_RS25955 (FYK18_26450) 44347..44937 + 591 WP_265451254.1 hypothetical protein -
FYK18_RS25960 44957..45087 - 131 Protein_59 DUF6404 family protein -
FYK18_RS25965 (FYK18_26455) 45075..45637 - 563 Protein_60 integrase core domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..132007 132007
- flank IS/Tn - - 45174..45455 281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11722.51 Da        Isoelectric Point: 6.4674

>T138131 WP_059242593.1 NZ_CP043270:c43276-42971 [Escherichia albertii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
SDLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T138131 NZ_CP057311:3395603-3395893 [Escherichia fergusonii]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACAAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT138131 WP_000813634.1 NZ_CP043270:c43496-43278 [Escherichia albertii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT138131 NZ_CP057311:3395371-3395613 [Escherichia fergusonii]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCAGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAGGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References