Detailed information of TA system TA138130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 31262..31863
• Replicon: plasmid p2RM9974
• Accession: NZ_CP043269
• Organism: Escherichia albertii strain RM9974

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: FYK18_RS25300
• Protein ID: WP_265451213.1
• Coordinates: 31483..31863 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A403D1Y2
• Locus tag: FYK18_RS25295
• Protein ID: WP_001190714.1
• Coordinates: 31262..31483 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK18_RS25250 (FYK18_25705)	26295..26753	+	459	WP_262937716.1	ead/Ea22-like family protein	-
FYK18_RS25255 (FYK18_25710)	26755..26952	+	198	WP_000360278.1	hypothetical protein	-
FYK18_RS25260 (FYK18_25715)	26963..28087	+	1125	WP_265451201.1	hypothetical protein	-
FYK18_RS25265 (FYK18_25720)	28133..28399	+	267	WP_265451203.1	hypothetical protein	-
FYK18_RS25270 (FYK18_25725)	28482..28895	+	414	WP_265451205.1	hypothetical protein	-
FYK18_RS25275 (FYK18_25730)	29644..30039	-	396	WP_265451206.1	hypothetical protein	-
FYK18_RS25280 (FYK18_25735)	30080..30286	-	207	WP_119047766.1	hypothetical protein	-
FYK18_RS25285	30469..30663	-	195	WP_265451208.1	hypothetical protein	-
FYK18_RS25290	30740..31039	-	300	WP_265451210.1	DNA-binding protein	-
FYK18_RS25295 (FYK18_25745)	31262..31483	+	222	WP_001190714.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
FYK18_RS25300 (FYK18_25750)	31483..31863	+	381	WP_265451213.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
FYK18_RS25305 (FYK18_25755)	31868..32047	+	180	WP_078182672.1	PdcA protein	-
FYK18_RS25310 (FYK18_25760)	32075..33118	+	1044	WP_265451215.1	DUF968 domain-containing protein	-
FYK18_RS25315 (FYK18_25765)	33207..33659	+	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
FYK18_RS25320 (FYK18_25770)	33746..34939	+	1194	WP_187187938.1	terminase	-
FYK18_RS25325 (FYK18_25775)	34939..36423	+	1485	WP_000124159.1	hypothetical protein	-
FYK18_RS25330	36637..36738	+	102	Protein_45	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..99091	99091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13630.38 Da
• Isoelectric Point: 5.1514

>T138130 WP_265451213.1 NZ_CP043269:31483-31863 [Escherichia albertii]
MRHISPEELIALHDANISRYGGLPGMSDPGRVEAIIGRVQARVAYEEISDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSVE

Nucleotide

• Length: 381 bp

>T138130 NZ_CP057311:c2610972-2610820 [Escherichia fergusonii]
ATGCCGCAGAAATACGGATTACTTTCGTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGAGAATTATGAGCTGGCGGCGTTTTTAGCCTGCAAATTGAAAGAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8123.06 Da
• Isoelectric Point: 4.7708

>AT138130 WP_001190714.1 NZ_CP043269:31262-31483 [Escherichia albertii]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGRESAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT138130 NZ_CP043269:31262-31483 [Escherichia albertii]
ATGCAGTCTATTAACTTCCGTACCGCACGCGGCAACCTTAGCGAAGTGCTCAATAATGTTGAAGCAGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAATCAGCCGTAATTGTCAGCAAGGCCACTTTCGAAGCCTATAAAAAAGCGGCGCTGGATG
CCGAATTTGCATCCCTGTTTGACACTCTGGACTCCACTAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A403D1Y2