Detailed information of TA system TA138095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 64222..64865
• Replicon: plasmid p1RM9976
• Accession: NZ_CP043265
• Organism: Escherichia albertii strain RM9976

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: FYK19_RS25790
• Protein ID: WP_059257121.1
• Coordinates: 64222..64638 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A5Z7X8E3
• Locus tag: FYK19_RS25795
• Protein ID: WP_001644959.1
• Coordinates: 64635..64865 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK19_RS25770 (60292)	60292..62133	-	1842	WP_265463472.1	DUF4158 domain-containing protein	-
FYK19_RS25775 (62290)	62290..62871	+	582	WP_001196199.1	recombinase family protein	-
FYK19_RS25780 (62878)	62878..63345	-	468	WP_265463473.1	transposase	-
FYK19_RS25785 (63398)	63398..64066	-	669	WP_059257120.1	class I SAM-dependent methyltransferase	-
FYK19_RS25790 (64222)	64222..64638	-	417	WP_059257121.1	type II toxin-antitoxin system VapC family toxin	Toxin
FYK19_RS25795 (64635)	64635..64865	-	231	WP_001644959.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FYK19_RS25800 (65151)	65151..65276	-	126	WP_262939952.1	hypothetical protein	-
FYK19_RS25805 (65417)	65417..65899	+	483	WP_237763820.1	MarC family protein	-
FYK19_RS25810 (66727)	66727..67677	+	951	WP_059257123.1	MBL fold metallo-hydrolase	-
FYK19_RS25815 (67681)	67681..67998	+	318	WP_059257124.1	hypothetical protein	-
FYK19_RS25820 (67986)	67986..68837	+	852	WP_059257125.1	DUF692 domain-containing protein	-
FYK19_RS25825 (68812)	68812..69525	+	714	WP_072247449.1	putative DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..200522	200522
	-	flank	IS/Tn	-	-	62878..63345	467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15007.36 Da
• Isoelectric Point: 8.5291

>T138095 WP_059257121.1 NZ_CP043265:c64638-64222 [Escherichia albertii]
VNKTYMLDTNICSFIMREQPEAVIRRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGQLVDAFCARLDAILPWDRA
AVDATVGVKAALTAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPNLVLEDWVK

Nucleotide

• Length: 417 bp

>T138095 NZ_CP043265:c64638-64222 [Escherichia albertii]
GTGAACAAAACGTATATGCTGGATACTAATATCTGCTCATTCATCATGCGTGAGCAGCCGGAGGCCGTGATCAGACGCTT
AGAGCAAGCGGTGCTGCGCAACCATCGCATCGTGGTCTCCGCTATTACCTACGCCGAAATGCGCTTCGGTGCTATCGGGA
AAAAAGCCTCGCCCCGTCATGGTCAACTGGTCGATGCGTTCTGCGCCCGCCTCGATGCGATTCTGCCGTGGGACCGTGCC
GCAGTCGATGCCACTGTAGGGGTCAAAGCAGCACTAACCGCAGCCGGTACACCAATCGGTCCCAACGACACAGCGATTGC
CGGACATGCTATAGCTGCAGGGGCCATTCTGGTGACCAATAATACGCGAGAGTTTGAGCGGGTGCCAAATTTGGTATTAG
AAGACTGGGTTAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8706.80 Da
• Isoelectric Point: 4.4692

>AT138095 WP_001644959.1 NZ_CP043265:c64865-64635 [Escherichia albertii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADADFLVEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT138095 NZ_CP057257:c90840-90619 [Escherichia fergusonii]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A5Z7X8E3