Detailed information of TA system TA138094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 55759..56318
• Replicon: plasmid p1RM9976
• Accession: NZ_CP043265
• Organism: Escherichia albertii strain RM9976

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: FYK19_RS25735
• Protein ID: WP_000421257.1
• Coordinates: 55759..56034 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FYK19_RS25740
• Protein ID: WP_072244091.1
• Coordinates: 56034..56318 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK19_RS25715 (51942)	51942..53169	+	1228	Protein_50	IS3 family transposase	-
FYK19_RS25720 (53647)	53647..54006	+	360	Protein_51	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
FYK19_RS25725 (54357)	54357..54971	-	615	WP_265463470.1	hypothetical protein	-
FYK19_RS25730 (55450)	55450..55653	-	204	Protein_53	hypothetical protein	-
FYK19_RS25735 (55759)	55759..56034	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FYK19_RS25740 (56034)	56034..56318	-	285	WP_072244091.1	ribbon-helix-helix domain-containing protein	Antitoxin
FYK19_RS25745 (56891)	56891..57256	+	366	WP_004106005.1	hypothetical protein	-
FYK19_RS25750 (57261)	57261..57356	+	96	Protein_57	hypothetical protein	-
FYK19_RS25755 (57398)	57398..58294	+	897	WP_262939980.1	hypothetical protein	-
FYK19_RS25760 (58340)	58340..59020	-	681	WP_262939979.1	3'-5' exonuclease	-
FYK19_RS25765 (59094)	59094..60344	-	1251	WP_265463471.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..200522	200522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T138094 WP_000421257.1 NZ_CP043265:c56034-55759 [Escherichia albertii]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T138094 NZ_CP057256:c2538430-2538278 [Escherichia fergusonii]
ATGCCGCAGAAATACGGATTACTTTCGTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGAGAGTTATGAGCTGGCGGCGTTTTTAGCCTGCAAATTGAAAGAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10498.00 Da
• Isoelectric Point: 7.2040

>AT138094 WP_072244091.1 NZ_CP043265:c56318-56034 [Escherichia albertii]
VKMKNNIAQTTKVLTAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDNVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT138094 NZ_CP043265:c56318-56034 [Escherichia albertii]
GTGAAAATGAAAAACAATATCGCACAAACGACAAAAGTGCTTACTGCGCATGTGCCATTACCTATGGCTGACAAAGTTGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACAATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCTTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5