Detailed information of TA system TA138091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 10058..10833
• Replicon: plasmid p1RM9976
• Accession: NZ_CP043265
• Organism: Escherichia albertii strain RM9976

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FYK19_RS25515
• Protein ID: WP_059257273.1
• Coordinates: 10351..10833 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: FYK19_RS25510
• Protein ID: WP_072146426.1
• Coordinates: 10058..10360 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK19_RS25480 (5333)	5333..5545	+	213	WP_001299730.1	ANR family transcriptional regulator	-
FYK19_RS25485 (5791)	5791..6141	+	351	Protein_4	thermonuclease family protein	-
FYK19_RS25490 (6176)	6176..6385	+	210	WP_265463467.1	hemolysin expression modulator Hha	-
FYK19_RS25495 (6590)	6590..7028	+	439	Protein_6	hypothetical protein	-
- (7123)	7123..7184	-	62	NuclAT_1	-	-
- (7123)	7123..7184	-	62	NuclAT_1	-	-
- (7123)	7123..7184	-	62	NuclAT_1	-	-
- (7123)	7123..7184	-	62	NuclAT_1	-	-
FYK19_RS25500 (7228)	7228..7398	+	171	WP_072247442.1	Hok/Gef family protein	-
FYK19_RS25505 (7965)	7965..9644	+	1680	WP_077871554.1	group II intron reverse transcriptase/maturase	-
FYK19_RS25510 (10058)	10058..10360	+	303	WP_072146426.1	DUF1778 domain-containing protein	Antitoxin
FYK19_RS25515 (10351)	10351..10833	+	483	WP_059257273.1	GNAT family N-acetyltransferase	Toxin
FYK19_RS25520 (11144)	11144..11398	+	255	WP_000083830.1	replication regulatory protein RepA	-
FYK19_RS25525 (11505)	11505..11682	+	178	Protein_12	protein CopA/IncA	-
FYK19_RS25530 (11701)	11701..12558	+	858	WP_265463468.1	incFII family plasmid replication initiator RepA	-
FYK19_RS25535 (13471)	13471..13740	+	270	WP_059272212.1	type II toxin-antitoxin system antitoxin YacA	-
FYK19_RS25540 (13740)	13740..14018	+	279	WP_262939943.1	type II toxin-antitoxin system toxin YacB	-
FYK19_RS25545 (14064)	14064..14617	+	554	Protein_16	3'-5' exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..200522	200522
	-	flank	IS/Tn	-	-	14805..15629	824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-99)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17650.45 Da
• Isoelectric Point: 7.4024

>T138091 WP_059257273.1 NZ_CP043265:10351-10833 [Escherichia albertii]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEGTLRVVGFYSLATGSVTHAELGRSLRHN
MPSPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVANLAEQVGIKAVMVHAIDDDARAFYERFGFVRSVVAPDTLFYKV

Nucleotide

• Length: 483 bp

>T138091 NZ_CP057256:c2507756-2507649 [Escherichia fergusonii]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCTGCTCCGGTCATTACTGGCATTCTTGCCAGTCTGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11648.21 Da
• Isoelectric Point: 4.7174

>AT138091 WP_072146426.1 NZ_CP043265:10058-10360 [Escherichia albertii]
MEVFMSTAASVRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT138091 NZ_CP057256:2507814-2507868 [Escherichia fergusonii]
CAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT