Detailed information of TA system TA138090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 27227..27786
• Replicon: plasmid p3RM9976
• Accession: NZ_CP043263
• Organism: Escherichia albertii strain RM9976

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: FYK19_RS24730
• Protein ID: WP_000421257.1
• Coordinates: 27227..27502 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FYK19_RS24735
• Protein ID: WP_265463349.1
• Coordinates: 27502..27786 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK19_RS24695 (FYK19_24980)	22942..23199	+	258	WP_000343085.1	hypothetical protein	-
FYK19_RS24700 (FYK19_24985)	23199..23789	+	591	WP_265463344.1	hypothetical protein	-
FYK19_RS24705 (FYK19_24990)	24002..25054	+	1053	WP_000655519.1	hypothetical protein	-
FYK19_RS24710 (FYK19_24995)	25230..25832	+	603	WP_097468803.1	ProQ/FINO family protein	-
FYK19_RS24715 (FYK19_25000)	25888..26382	+	495	WP_265463347.1	hypothetical protein	-
FYK19_RS24720 (FYK19_25005)	26382..26648	+	267	WP_021576094.1	hypothetical protein	-
FYK19_RS24725 (FYK19_25015)	26918..27121	-	204	Protein_38	hypothetical protein	-
FYK19_RS24730 (FYK19_25020)	27227..27502	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FYK19_RS24735 (FYK19_25025)	27502..27786	-	285	WP_265463349.1	ribbon-helix-helix domain-containing protein	Antitoxin
FYK19_RS24740 (FYK19_25040)	28690..29562	-	873	WP_045145554.1	incFII family plasmid replication initiator RepA	-
FYK19_RS24745	30526..30684	+	159	Protein_42	ash family protein	-
FYK19_RS24750 (FYK19_25065)	30722..31009	+	288	WP_000074855.1	conjugal transfer protein TraA	-
FYK19_RS24755 (FYK19_25075)	31451..31984	+	534	WP_001372168.1	transcription termination/antitermination NusG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..84014	84014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T138090 WP_000421257.1 NZ_CP043263:c27502-27227 [Escherichia albertii]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T138090 NZ_CP057256:c1826601-1826194 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGCTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACTTGTGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.89 Da
• Isoelectric Point: 5.6558

>AT138090 WP_265463349.1 NZ_CP043263:c27786-27502 [Escherichia albertii]
MQMKNNSTQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT138090 NZ_CP043263:c27786-27502 [Escherichia albertii]
ATGCAAATGAAAAACAATAGTACACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5