Detailed information of TA system TA138085

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4866770..4867477
• Replicon: chromosome
• Accession: NZ_CP043262
• Organism: Escherichia albertii strain RM9976

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: FYK19_RS24030
• Protein ID: WP_161537416.1
• Coordinates: 4867136..4867477 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: FYK19_RS24025
• Protein ID: WP_000939437.1
• Coordinates: 4866770..4867105 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK19_RS23995 (4862719)	4862719..4862937	-	219	WP_059219068.1	AlpA family phage regulatory protein	-
FYK19_RS24000 (4863055)	4863055..4863669	-	615	WP_161537419.1	inovirus Gp2 family protein	-
FYK19_RS24005 (4864262)	4864262..4864906	+	645	WP_237579909.1	hypothetical protein	-
FYK19_RS24010 (4864966)	4864966..4865214	+	249	WP_233991479.1	hypothetical protein	-
FYK19_RS24015 (4865450)	4865450..4866267	+	818	Protein_4685	DUF932 domain-containing protein	-
FYK19_RS24020 (4866297)	4866297..4866770	+	474	WP_265462389.1	DNA repair protein RadC	-
FYK19_RS24025 (4866770)	4866770..4867105	+	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FYK19_RS24030 (4867136)	4867136..4867477	+	342	WP_161537416.1	TA system toxin CbtA family protein	Toxin
FYK19_RS24035 (4867592)	4867592..4868425	+	834	WP_161537415.1	DUF4942 domain-containing protein	-
FYK19_RS24040 (4868502)	4868502..4868750	+	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
FYK19_RS24045 (4868754)	4868754..4869029	+	276	WP_161537414.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FYK19_RS24050 (4869148)	4869148..4869939	+	792	WP_161537413.1	helix-turn-helix transcriptional regulator	-
FYK19_RS24055 (4870211)	4870211..4871194	+	984	WP_161537412.1	restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	vat / fimB / fimE	4864966..4885648	20682

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13049.02 Da
• Isoelectric Point: 9.5454

>T138085 WP_161537416.1 NZ_CP043262:4867136-4867477 [Escherichia albertii]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGQLRRCHNTTAR

Nucleotide

• Length: 342 bp

>T138085 NZ_CP043262:4867136-4867477 [Escherichia albertii]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGACTGAACCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTTGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGAGGCAGGCTACAGGTCAGCTGCGTCGCTG
CCATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT138085 WP_000939437.1 NZ_CP043262:4866770-4867105 [Escherichia albertii]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT138085 NZ_CP057256:c483650-483366 [Escherichia fergusonii]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTTACCGGAAAAGCGGCATTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTAGATGTGTCGCGACTACGCCGTCTGGTTACGCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6