Detailed information of TA system TA138061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 33453..34096
• Replicon: plasmid p1RM10507
• Accession: NZ_CP043261
• Organism: Escherichia albertii strain RM10507

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: FYK20_RS25705
• Protein ID: WP_124783386.1
• Coordinates: 33680..34096 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A5Z7X8E3
• Locus tag: FYK20_RS25700
• Protein ID: WP_001644959.1
• Coordinates: 33453..33683 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK20_RS25675 (28792)	28792..29505	-	714	WP_265464789.1	putative DNA-binding domain-containing protein	-
FYK20_RS25680 (29480)	29480..30331	-	852	WP_265464790.1	DUF692 domain-containing protein	-
FYK20_RS25685 (30319)	30319..30636	-	318	WP_059236668.1	hypothetical protein	-
FYK20_RS25690 (30640)	30640..31590	-	951	WP_265464791.1	MBL fold metallo-hydrolase	-
FYK20_RS25695 (32418)	32418..32900	-	483	WP_265464792.1	MarC family protein	-
FYK20_RS25700 (33453)	33453..33683	+	231	WP_001644959.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FYK20_RS25705 (33680)	33680..34096	+	417	WP_124783386.1	type II toxin-antitoxin system VapC family toxin	Toxin
FYK20_RS25710 (34252)	34252..35061	+	810	WP_059278860.1	class I SAM-dependent methyltransferase	-
FYK20_RS25715 (35737)	35737..35966	+	230	Protein_29	type II toxin-antitoxin system VapB family antitoxin	-
FYK20_RS25720 (35963)	35963..36299	+	337	Protein_30	VapC toxin family PIN domain ribonuclease	-
FYK20_RS25725 (36349)	36349..38301	-	1953	WP_113650881.1	sigma 54-interacting transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	vat	1..102034	102034

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15051.37 Da
• Isoelectric Point: 7.2447

>T138061 WP_124783386.1 NZ_CP043261:33680-34096 [Escherichia albertii]
VNKTYMLDTNICSFIMREQPEAVIRRLEQAVLRNHRIVVSAITYAEMRFGAIGKKASPRHGQLVDAFCARLDAILPWDRA
AVDATVEVKAALTAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFEQVPNLVLEDWVK

Nucleotide

• Length: 417 bp

>T138061 NZ_CP057243:760618-760932 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCATTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8706.80 Da
• Isoelectric Point: 4.4692

>AT138061 WP_001644959.1 NZ_CP043261:33453-33683 [Escherichia albertii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADADFLVEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT138061 NZ_CP043261:33453-33683 [Escherichia albertii]
ATGCGAACAGTATCTATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCTCGCGATCTGGATTTTGAGGGGGTGAG
TGAGTTGGAGATTGTCCGCGAAGGGGACAGCATCATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
AAAAGGCCGACGCAGACTTTTTGGTGGAGCGTGAAGACGTGGTCAGTGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5Z7X8E3