Detailed information of TA system TA138059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 28140..28665
• Replicon: plasmid p3RM10507
• Accession: NZ_CP043259
• Organism: Escherichia albertii strain RM10507

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: FYK20_RS24965
• Protein ID: WP_113650864.1
• Coordinates: 28360..28665 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: FYK20_RS24960
• Protein ID: WP_113650865.1
• Coordinates: 28140..28358 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK20_RS24940 (25118)	25118..26047	-	930	WP_240645351.1	IS21-like element helper ATPase IstB	-
FYK20_RS24945 (25972)	25972..26160	-	189	WP_164508686.1	hypothetical protein	-
FYK20_RS24950 (26712)	26712..27308	-	597	WP_113650867.1	hypothetical protein	-
FYK20_RS24955 (27296)	27296..27544	-	249	WP_113650866.1	hypothetical protein	-
FYK20_RS24960 (28140)	28140..28358	+	219	WP_113650865.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
FYK20_RS24965 (28360)	28360..28665	+	306	WP_113650864.1	type II toxin-antitoxin system toxin CcdB	Toxin
FYK20_RS24970 (28666)	28666..29475	+	810	WP_113650863.1	site-specific integrase	-
FYK20_RS24975 (29606)	29606..29881	-	276	WP_000239529.1	hypothetical protein	-
FYK20_RS24980 (29875)	29875..30519	-	645	WP_000633916.1	division plane positioning ATPase MipZ	-
FYK20_RS24985 (30742)	30742..31713	+	972	WP_001103697.1	plasmid segregation protein ParM	-
FYK20_RS24990 (31718)	31718..32110	+	393	WP_000340830.1	plasmid partitioning/stability family protein	-
FYK20_RS24995 (32115)	32115..33384	-	1270	Protein_44	translesion error-prone DNA polymerase V subunit UmuC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42515	42515
	-	flank	IS/Tn	-	-	25118..26047	929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11687.47 Da
• Isoelectric Point: 6.2213

>T138059 WP_113650864.1 NZ_CP043259:28360-28665 [Escherichia albertii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAHLLSDKVSRELYPVVHVGDESWRMMTTDMASVPISVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T138059 NZ_CP043259:28360-28665 [Escherichia albertii]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTAGCCAGTGCCCACCTGCTGTCAGATAAGGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTTGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGATCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8310.30 Da
• Isoelectric Point: 5.0338

>AT138059 WP_113650865.1 NZ_CP043259:28140-28358 [Escherichia albertii]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAVRWKAENQEGMAEVARFIEVNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT138059 NZ_CP057243:c584963-584628 [Escherichia fergusonii]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGGCTGGACAGCATTCATTACGAAATTCTACCTGGAGGGCAAGTATTTA
TGTGCCGATTGGGAGATGAACAGGAGGATCCTACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA