Detailed information of TA system TA138058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 7769..8478
• Replicon: plasmid p3RM10507
• Accession: NZ_CP043259
• Organism: Escherichia albertii strain RM10507

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A1C7CTN8
• Locus tag: FYK20_RS24840
• Protein ID: WP_001420428.1
• Coordinates: 7996..8478 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: FYK20_RS24835
• Protein ID: WP_265464718.1
• Coordinates: 7769..8005 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYK20_RS24805 (2979)	2979..3212	+	234	WP_000005990.1	DUF905 family protein	-
FYK20_RS24810 (3278)	3278..5215	+	1938	WP_125295810.1	ParB/RepB/Spo0J family partition protein	-
FYK20_RS24815 (5270)	5270..5704	+	435	WP_016239106.1	conjugation system SOS inhibitor PsiB	-
FYK20_RS24820 (5701)	5701..6463	+	763	Protein_9	plasmid SOS inhibition protein A	-
FYK20_RS24825 (6432)	6432..6620	-	189	WP_060565194.1	hypothetical protein	-
- (6432)	6432..6656	+	225	NuclAT_0	-	-
- (6432)	6432..6656	+	225	NuclAT_0	-	-
- (6432)	6432..6656	+	225	NuclAT_0	-	-
- (6432)	6432..6656	+	225	NuclAT_0	-	-
FYK20_RS24830 (6656)	6656..7729	-	1074	WP_099588292.1	integrase core domain-containing protein	-
FYK20_RS24835 (7769)	7769..8005	+	237	WP_265464718.1	DUF1778 domain-containing protein	Antitoxin
FYK20_RS24840 (7996)	7996..8478	+	483	WP_001420428.1	GNAT family N-acetyltransferase	Toxin
FYK20_RS24845 (8763)	8763..9020	+	258	WP_000083826.1	replication regulatory protein RepA	-
FYK20_RS24850 (9255)	9255..9329	+	75	WP_001365705.1	RepA leader peptide Tap	-
FYK20_RS24855 (9322)	9322..10179	+	858	WP_062897587.1	incFII family plasmid replication initiator RepA	-
FYK20_RS24860 (11112)	11112..11219	+	108	Protein_17	XRE family transcriptional regulator	-
FYK20_RS24865 (11239)	11239..11502	+	264	WP_113650841.1	plasmid stabilization protein	-
FYK20_RS24870 (11511)	11511..11834	-	324	Protein_19	recombinase family protein	-
FYK20_RS24875 (11907)	11907..12545	-	639	WP_113650840.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42515	42515
	-	inside	IScluster/Tn	-	-	6656..11825	5169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-72)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17728.46 Da
• Isoelectric Point: 6.5974

>T138058 WP_001420428.1 NZ_CP043259:7996-8478 [Escherichia albertii]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQILNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVANLAEQVGIKAVMVHAIDDDARVFYEHFGFVQSVVAPDTLFYKI

Nucleotide

• Length: 483 bp

>T138058 NZ_CP057243:540154-540444 [Escherichia fergusonii]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACAAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTGATCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9006.25 Da
• Isoelectric Point: 4.7361

>AT138058 WP_265464718.1 NZ_CP043259:7769-8005 [Escherichia albertii]
ISVNLHRAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDNEGYDSFITQLEAPVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 237 bp

>AT138058 NZ_CP043259:7769-8005 [Escherichia albertii]
ATCTCTGTGAACTTACACAGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAATCGTACCGATTTCATCATTGA
ACTGGCTTACCAGGAAGCTAAAAACATCATTCTGGATCAACGTTTATTTGTATTGGATAACGAAGGTTATGATTCGTTTA
TTACGCAACTGGAAGCTCCTGTACAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A1C7CTN8