Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3248074..3248725 Replicon chromosome
Accession NZ_CP043258
Organism Escherichia albertii strain RM10507

Toxin (Protein)


Gene name cptA Uniprot ID B1EG01
Locus tag FYK20_RS16175 Protein ID WP_000244763.1
Coordinates 3248074..3248478 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FYK20_RS16180 Protein ID WP_000354046.1
Coordinates 3248459..3248725 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYK20_RS16155 (3244032) 3244032..3245765 - 1734 WP_000813238.1 single-stranded-DNA-specific exonuclease RecJ -
FYK20_RS16160 (3245771) 3245771..3246481 - 711 WP_000748106.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FYK20_RS16165 (3246506) 3246506..3247402 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FYK20_RS16170 (3247514) 3247514..3248035 + 522 WP_001055879.1 flavodoxin FldB -
FYK20_RS16175 (3248074) 3248074..3248478 - 405 WP_000244763.1 protein YgfX Toxin
FYK20_RS16180 (3248459) 3248459..3248725 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FYK20_RS16185 (3248715) 3248715..3248945 - 231 WP_000181267.1 hypothetical protein -
FYK20_RS16190 (3248977) 3248977..3249957 + 981 WP_000886056.1 tRNA-modifying protein YgfZ -
FYK20_RS16195 (3250159) 3250159..3250818 - 660 WP_000250281.1 hemolysin III family protein -
FYK20_RS16200 (3250902) 3250902..3251936 - 1035 Protein_3159 IS481 family transposase -
FYK20_RS16205 (3252087) 3252087..3252398 - 312 WP_059258658.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3250716..3251936 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15888.81 Da        Isoelectric Point: 11.1732

>T138051 WP_000244763.1 NZ_CP043258:c3248478-3248074 [Escherichia albertii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAAILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVRAPWMIKTGMMLRLLSDSGKRQHLWLAADSMDEAEWRDLRRILLQQEIQ

Download         Length: 405 bp

>T138051 NZ_CP057239:c75457-75251 [Escherichia fergusonii]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGTTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT138051 WP_000354046.1 NZ_CP043258:c3248725-3248459 [Escherichia albertii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT138051 NZ_CP057239:75444-75505 [Escherichia fergusonii]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S6P9B3

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References