Detailed information of TA system TA138038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2359307..2360285
• Replicon: chromosome
• Accession: NZ_CP043234
• Organism: Bacillus thuringiensis strain Bt-GS57

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: FM050_RS15990
• Protein ID: WP_000624977.1
• Coordinates: 2359307..2360044 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: FM050_RS15995
• Protein ID: WP_000237818.1
• Coordinates: 2360157..2360285 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FM050_RS15970 (FM050_16320)	2354950..2355732	+	783	WP_000381800.1	class I SAM-dependent methyltransferase	-
FM050_RS15975 (FM050_16325)	2355890..2357575	-	1686	WP_003284281.1	alpha-keto acid decarboxylase family protein	-
FM050_RS15980 (FM050_16330)	2357683..2358165	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
FM050_RS15985 (FM050_16335)	2358332..2359069	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
FM050_RS15990 (FM050_16340)	2359307..2360044	+	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
FM050_RS15995 (FM050_16345)	2360157..2360285	+	129	WP_000237818.1	hypothetical protein	Antitoxin
FM050_RS16000 (FM050_16350)	2360358..2360534	+	177	WP_000852629.1	stage II sporulation protein SB	-
FM050_RS16005 (FM050_16355)	2360553..2360942	-	390	WP_000713866.1	YxeA family protein	-
FM050_RS16010 (FM050_16360)	2361154..2362623	+	1470	WP_000287515.1	beta-Ala-His dipeptidase	-
FM050_RS16015 (FM050_16365)	2362869..2363678	+	810	WP_000678509.1	papain-like cysteine protease family protein	-
FM050_RS16020 (FM050_16370)	2363704..2364306	+	603	WP_000517260.1	hypothetical protein	-
FM050_RS16025 (FM050_16375)	2364546..2364798	-	253	Protein_2331	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T138038 WP_000624977.1 NZ_CP043234:2359307-2360044 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T138038 NZ_CP057230:136809-137225 [Escherichia fergusonii]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT138038 WP_000237818.1 NZ_CP043234:2360157-2360285 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT138038 NZ_CP057230:136582-136812 [Escherichia fergusonii]
ATGAGAACTGTGTCTATTTTTAAAAACGGCAATAACCGTGCTATCCGTCTGCCCCGAGAACTGGATTTTGAGGGGGTAAG
CGAGCTCGAAATAATCCGGGAAGGAAACAGCATTATCCTGCGTCCTGTCCGGCCTACCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5