Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 234379..235021 Replicon chromosome
Accession NZ_CP043234
Organism Bacillus thuringiensis strain Bt-GS57

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag FM050_RS05150 Protein ID WP_000635965.1
Coordinates 234671..235021 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FM050_RS05145 Protein ID WP_000004570.1
Coordinates 234379..234666 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FM050_RS05120 (FM050_05340) 229696..230658 + 963 WP_000961168.1 UV DNA damage repair endonuclease UvsE -
FM050_RS05125 (FM050_05345) 230651..231223 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
FM050_RS05130 (FM050_05350) 231317..231676 + 360 WP_000583417.1 holo-ACP synthase -
FM050_RS05135 (FM050_05355) 231833..232783 + 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
FM050_RS05140 (FM050_05360) 232901..234070 + 1170 WP_000390616.1 alanine racemase -
FM050_RS05145 (FM050_05365) 234379..234666 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FM050_RS05150 (FM050_05370) 234671..235021 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FM050_RS05155 (FM050_05375) 235089..237257 + 2169 WP_000426236.1 Tex family protein -
FM050_RS05160 (FM050_05380) 237316..237432 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FM050_RS05165 (FM050_05385) 237628..238086 + 459 WP_000344239.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T138037 WP_000635965.1 NZ_CP043234:234671-235021 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T138037 NZ_CP057230:133550-133966 [Escherichia fergusonii]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGGCGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT138037 WP_000004570.1 NZ_CP043234:234379-234666 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT138037 NZ_CP043234:234379-234666 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References