Detailed information of TA system TA138033

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 5573..6315
• Replicon: plasmid unnamed
• Accession: NZ_CP043231
• Organism: Bacillus thuringiensis strain Bt-GS57

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: FM050_RS00030
• Protein ID: WP_016097319.1
• Coordinates: 5731..6315 (+)
• Length: 195 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	5573..5669 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FM050_RS00010 (1123)	1123..2754	+	1632	WP_016097315.1	ArdC-like ssDNA-binding domain-containing protein	-
FM050_RS00015 (2816)	2816..3802	+	987	WP_016097316.1	hypothetical protein	-
FM050_RS00020 (3824)	3824..4375	+	552	WP_016097317.1	metal-dependent hydrolase	-
FM050_RS00025 (4455)	4455..5480	+	1026	WP_016097318.1	DUF3991 and TOPRIM domain-containing protein	-
- (5573)	5573..5669	+	97	NuclAT_0	-	Antitoxin
- (5573)	5573..5669	+	97	NuclAT_0	-	Antitoxin
- (5573)	5573..5669	+	97	NuclAT_0	-	Antitoxin
- (5573)	5573..5669	+	97	NuclAT_0	-	Antitoxin
FM050_RS00030 (5731)	5731..6315	+	585	WP_016097319.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
FM050_RS00035 (6383)	6383..7684	+	1302	WP_016097320.1	hypothetical protein	-
FM050_RS00040 (7791)	7791..8171	+	381	WP_016097321.1	hypothetical protein	-
FM050_RS00045 (8292)	8292..10433	+	2142	WP_016097322.1	type IA DNA topoisomerase	-
FM050_RS00050 (10463)	10463..10909	+	447	WP_016097323.1	CPBP family intramembrane metalloprotease	-
FM050_RS00055 (11062)	11062..11232	-	171	WP_016097324.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..76132	76132

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-169)

Sequences

Toxin

Protein

• Length: 195 a.a.
• Molecular weight: 22900.17 Da
• Isoelectric Point: 8.9694

>T138033 WP_016097319.1 NZ_CP043231:5731-6315 [Bacillus thuringiensis]
MANKDNPKFYTISTEYIDYLRETDSKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDTDEKRIG
VLLVNNMIPVPEKECKEIDIAEKTAADPQYGNLMLKQYLFLKENMDRVTNKVEKVYKDVTVQGKPSHKQKFLKGVCCDFP
KLEEKCQEYKERDQAKERDKARRIAYMRQMGRER

Nucleotide

• Length: 585 bp

>T138033 NZ_CP057230:c75457-75251 [Escherichia fergusonii]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGTTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT138033 NZ_CP043231:5573-5669 [Bacillus thuringiensis]
GTAACCTTACTTTAGTAGGTGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCGCTAAAAAA

Structures

Antitoxin