Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 44319..44844 Replicon plasmid pEc-050-NDM-5
Accession NZ_CP043230
Organism Escherichia coli strain Ec-050

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FX998_RS24955 Protein ID WP_001159871.1
Coordinates 44539..44844 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag FX998_RS24950 Protein ID WP_000813630.1
Coordinates 44319..44537 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FX998_RS24920 39529..40215 + 687 Protein_41 IS1 family transposase -
FX998_RS24925 40469..41491 - 1023 WP_000361402.1 helicase UvrD -
FX998_RS24930 41476..43041 - 1566 WP_001128474.1 AAA family ATPase -
FX998_RS24935 43116..43532 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
FX998_RS24940 43529..43759 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FX998_RS24950 44319..44537 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FX998_RS24955 44539..44844 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FX998_RS24960 44845..45651 + 807 WP_000016970.1 site-specific integrase -
FX998_RS24965 46373..47128 + 756 WP_000852146.1 replication initiation protein RepE -
FX998_RS24970 47716..48882 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IIa / mph(A) / sitABCD / tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 iucA / iucB / iucC / iucD / iutA 1..99461 99461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T138028 WP_001159871.1 NZ_CP043230:44539-44844 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T138028 NZ_CP043230:44539-44844 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT138028 WP_000813630.1 NZ_CP043230:44319-44537 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT138028 NZ_CP057229:3468548-3468605 [Escherichia fergusonii]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References