Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 43116..43759 Replicon plasmid pEc-050-NDM-5
Accession NZ_CP043230
Organism Escherichia coli strain Ec-050

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag FX998_RS24935 Protein ID WP_001034044.1
Coordinates 43116..43532 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag FX998_RS24940 Protein ID WP_001261286.1
Coordinates 43529..43759 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FX998_RS24920 39529..40215 + 687 Protein_41 IS1 family transposase -
FX998_RS24925 40469..41491 - 1023 WP_000361402.1 helicase UvrD -
FX998_RS24930 41476..43041 - 1566 WP_001128474.1 AAA family ATPase -
FX998_RS24935 43116..43532 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
FX998_RS24940 43529..43759 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FX998_RS24950 44319..44537 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
FX998_RS24955 44539..44844 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
FX998_RS24960 44845..45651 + 807 WP_000016970.1 site-specific integrase -
FX998_RS24965 46373..47128 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IIa / mph(A) / sitABCD / tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 iucA / iucB / iucC / iucD / iutA 1..99461 99461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T138027 WP_001034044.1 NZ_CP043230:c43532-43116 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T138027 NZ_CP057229:c2533095-2532988 [Escherichia fergusonii]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT138027 WP_001261286.1 NZ_CP043230:c43759-43529 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT138027 NZ_CP057229:2533143-2533209 [Escherichia fergusonii]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References