Detailed information of TA system TA138008

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2157631..2158463
• Replicon: chromosome
• Accession: NZ_CP043227
• Organism: Escherichia coli strain Ec-050

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: FX998_RS10570
• Protein ID: WP_000854765.1
• Coordinates: 2158089..2158463 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: FX998_RS10565
• Protein ID: WP_001295723.1
• Coordinates: 2157631..2157999 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FX998_RS10530	2153295..2153975	+	681	WP_001282921.1	WYL domain-containing protein	-
FX998_RS10535	2154123..2154800	+	678	WP_001097302.1	hypothetical protein	-
FX998_RS10540	2154806..2155039	+	234	WP_001278293.1	DUF905 family protein	-
FX998_RS10545	2155129..2155947	+	819	WP_001175165.1	DUF945 domain-containing protein	-
FX998_RS25425	2155973..2156113	-	141	WP_000997937.1	hypothetical protein	-
FX998_RS10550	2156213..2156692	+	480	WP_057109541.1	antirestriction protein	-
FX998_RS10555	2156708..2157184	+	477	WP_001186774.1	RadC family protein	-
FX998_RS10560	2157247..2157468	+	222	WP_000692341.1	DUF987 domain-containing protein	-
FX998_RS10565	2157631..2157999	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FX998_RS10570	2158089..2158463	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
FX998_RS10575	2158463..2158666	+	204	WP_001398797.1	hypothetical protein	-
FX998_RS10580	2158691..2158885	+	195	WP_024181941.1	DUF957 domain-containing protein	-
FX998_RS10585	2158970..2159812	+	843	WP_022645115.1	DUF4942 domain-containing protein	-
FX998_RS10590	2160093..2160629	-	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
FX998_RS10595	2160631..2161809	-	1179	WP_000094991.1	type II secretion system protein GspL	-
FX998_RS10600	2161806..2162783	-	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
FX998_RS10605	2162786..2163385	-	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T138008 WP_000854765.1 NZ_CP043227:2158089-2158463 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T138008 NZ_CP057220:1200555-1200857 [Escherichia fergusonii]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCACTCAACGCCACATAGAAA
GATTCCTGCTGAAATCCATACCACATTATCAAGAAAATTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTATTTAGATCCACACAACTACTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT138008 WP_001295723.1 NZ_CP043227:2157631-2157999 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT138008 NZ_CP043227:2157631-2157999 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52