Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2021094..2021748 Replicon chromosome
Accession NZ_CP043227
Organism Escherichia coli strain Ec-050

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FX998_RS09830 Protein ID WP_000244777.1
Coordinates 2021094..2021501 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FX998_RS09835 Protein ID WP_000354046.1
Coordinates 2021482..2021748 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FX998_RS09810 2017051..2018784 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
FX998_RS09815 2018790..2019500 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FX998_RS09820 2019525..2020421 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FX998_RS09825 2020533..2021054 + 522 WP_001055874.1 flavodoxin FldB -
FX998_RS09830 2021094..2021501 - 408 WP_000244777.1 toxin CptA Toxin
FX998_RS09835 2021482..2021748 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FX998_RS09840 2021991..2022971 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FX998_RS09845 2023167..2023826 - 660 WP_000250274.1 hemolysin III family protein -
FX998_RS09850 2023990..2024301 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
FX998_RS09855 2024346..2025779 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
FX998_RS09860 2025836..2026579 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T138007 WP_000244777.1 NZ_CP043227:c2021501-2021094 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T138007 NZ_CP057220:983786-983938 [Escherichia fergusonii]
ATGCTGACAAAATATGCCCTTGTGGCAATCATCGTACTGTGTTGTACAGTACTGGGATTCACGCTGATGGTAGGCGACTC
GTTGTGTGAGTTGAGTATCAGAGAACGTGGTATGGAGTTTAAGGCAGTTCTCGCTTACGAATCGAAGAAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT138007 WP_000354046.1 NZ_CP043227:c2021748-2021482 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT138007 NZ_CP057220:c983744-983692 [Escherichia fergusonii]
TTAGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References