Detailed information of TA system TA138004

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1033425..1034260
• Replicon: chromosome
• Accession: NZ_CP043227
• Organism: Escherichia coli strain Ec-050

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: FX998_RS05175
• Protein ID: WP_042046912.1
• Coordinates: 1033883..1034260 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FX998_RS05170
• Protein ID: WP_135145692.1
• Coordinates: 1033425..1033793 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FX998_RS05135	1029085..1029332	+	248	Protein_990	antirestriction protein	-
FX998_RS05140	1029427..1029900	+	474	WP_000855059.1	antirestriction protein	-
FX998_RS05145	1029916..1030368	+	453	WP_129445215.1	RadC family protein	-
FX998_RS05150	1030509..1032050	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
FX998_RS05155	1032065..1032811	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
FX998_RS05160	1032862..1032978	+	117	WP_143422743.1	hypothetical protein	-
FX998_RS05165	1033041..1033262	+	222	WP_000692345.1	DUF987 domain-containing protein	-
FX998_RS05170	1033425..1033793	+	369	WP_135145692.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FX998_RS05175	1033883..1034260	+	378	WP_042046912.1	TA system toxin CbtA family protein	Toxin
FX998_RS05180	1034257..1034460	+	204	WP_000761705.1	hypothetical protein	-
FX998_RS05190	1034745..1035773	+	1029	Protein_1000	IS110 family transposase	-
FX998_RS05195	1036044..1036214	+	171	Protein_1001	propanediol utilization protein	-
FX998_RS05200	1036227..1036409	+	183	WP_024198358.1	ethanolamine utilization protein	-
FX998_RS05205	1036510..1036839	-	330	WP_000450409.1	DUF496 family protein	-
FX998_RS05210	1037011..1038069	-	1059	WP_001200895.1	FUSC family protein	-
FX998_RS05215	1038267..1038740	-	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14020.96 Da
• Isoelectric Point: 7.8276

>T138004 WP_042046912.1 NZ_CP043227:1033883-1034260 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T138004 NZ_CP043227:1033883-1034260 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13545.30 Da
• Isoelectric Point: 6.4764

>AT138004 WP_135145692.1 NZ_CP043227:1033425-1033793 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPHHQHTVTLYAKGLTCEADTLGSCGYAYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT138004 NZ_CP043227:1033425-1033793 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCACCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA