Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 412567..413205 Replicon chromosome
Accession NZ_CP043227
Organism Escherichia coli strain Ec-050

Toxin (Protein)


Gene name hicA Uniprot ID A0A0A6TXU8
Locus tag FX998_RS02140 Protein ID WP_001447010.1
Coordinates 412567..412743 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FX998_RS02145 Protein ID WP_001270286.1
Coordinates 412789..413205 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FX998_RS02120 408186..409400 - 1215 WP_001326689.1 BenE family transporter YdcO -
FX998_RS02125 409453..409989 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FX998_RS02130 410062..412023 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FX998_RS02135 412115..412345 - 231 WP_000494244.1 YncJ family protein -
FX998_RS02140 412567..412743 + 177 WP_001447010.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FX998_RS02145 412789..413205 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FX998_RS02150 413284..414690 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
FX998_RS02155 414935..416080 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
FX998_RS02160 416098..417111 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
FX998_RS02165 417112..418053 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6779.86 Da        Isoelectric Point: 11.2298

>T137994 WP_001447010.1 NZ_CP043227:412567-412743 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T137994 NZ_CP043227:412567-412743 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT137994 WP_001270286.1 NZ_CP043227:412789-413205 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT137994 NZ_CP057215:4462582-4462648 [Escherichia fergusonii]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6TXU8

Antitoxin

Source ID Structure

References