Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1771..2318 Replicon plasmid pET6.2-IncFII
Accession NZ_CP043219
Organism Escherichia coli O80:H26 strain EC-107

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag FYA02_RS25850 Protein ID WP_000969996.1
Coordinates 2037..2318 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag FYA02_RS25845 Protein ID WP_000079928.1
Coordinates 1771..2040 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA02_RS25835 1..858 + 858 WP_000130962.1 incFII family plasmid replication initiator RepA -
FYA02_RS27135 1558..1716 + 159 WP_000841008.1 hypothetical protein -
FYA02_RS25845 1771..2040 + 270 WP_000079928.1 hypothetical protein Antitoxin
FYA02_RS25850 2037..2318 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FYA02_RS25855 2358..3206 + 849 WP_001057996.1 3'-5' exonuclease -
FYA02_RS25860 3524..4177 + 654 Protein_6 helix-turn-helix domain-containing protein -
FYA02_RS25865 4186..5046 + 861 Protein_7 EamA family transporter -
FYA02_RS25870 5078..6277 - 1200 WP_000804064.1 tetracycline efflux MFS transporter Tet(A) -
FYA02_RS25875 6383..7033 + 651 WP_000164043.1 tetracycline resistance transcriptional repressor TetR(A) -
FYA02_RS25880 7065..7307 - 243 WP_000844627.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / aph(3'')-Ib / aph(6)-Id - 1..94175 94175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T137963 WP_000969996.1 NZ_CP043219:2037-2318 [Escherichia coli O80:H26]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T137963 NZ_CP043219:2037-2318 [Escherichia coli O80:H26]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT137963 WP_000079928.1 NZ_CP043219:1771-2040 [Escherichia coli O80:H26]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT137963 NZ_CP057210:c1176899-1176579 [Escherichia fergusonii]
ATGAAAAATTCATGGGGACTGAAGCGCGACATGACGCCGTGCTTCGGTGCAAGGCTTGTGCAGGAAGGCCGTCGCCTGCA
TTTTCTGGCGGACCGTGCAGATATTAGCGGTGCGTTTAATGAGGAGGAGGCGCTACGTCTGGATCAAGCATTTCCGCTAG
TGATGAAACAGCTTGAATTGATGCTGACAACCGGTGAACTAAATCCACGCCATCAGCATTGCGTCACGTTGCATCATAAC
GGACTCACCTGCGAGGCGGATACTCTCGGCTCTCATGGCTACGTTTACATCGTTATTTATCCTCAGACATCGAGCCAGTA
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References