Detailed information of TA system TA137962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 71992..72593
• Replicon: plasmid pET6.1-IncY
• Accession: NZ_CP043218
• Organism: Escherichia coli O80:H26 strain EC-107

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: FYA02_RS25635
• Protein ID: WP_137471994.1
• Coordinates: 71992..72372 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: FYA02_RS25640
• Protein ID: WP_001190712.1
• Coordinates: 72372..72593 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA02_RS25610	67433..68917	-	1485	WP_000124150.1	terminase	-
FYA02_RS25615	68917..70110	-	1194	WP_148880110.1	terminase	-
FYA02_RS25620	70196..70648	-	453	WP_001326849.1	late promoter-activating protein	-
FYA02_RS25625	70737..71780	-	1044	WP_148880111.1	DUF968 domain-containing protein	-
FYA02_RS25630	71808..71987	-	180	WP_001339207.1	hypothetical protein	-
FYA02_RS25635	71992..72372	-	381	WP_137471994.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
FYA02_RS25640	72372..72593	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FYA02_RS25645	72776..74332	+	1557	WP_148880117.1	type I restriction-modification system subunit M	-
FYA02_RS25650	74329..75549	+	1221	WP_148880112.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / blaTEM-1B	-	1..102028	102028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13687.38 Da
• Isoelectric Point: 4.8945

>T137962 WP_137471994.1 NZ_CP043218:c72372-71992 [Escherichia coli O80:H26]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVEAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T137962 NZ_CP043218:c72372-71992 [Escherichia coli O80:H26]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCTGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAATGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGAGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT137962 WP_001190712.1 NZ_CP043218:c72593-72372 [Escherichia coli O80:H26]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT137962 NZ_CP043218:c72593-72372 [Escherichia coli O80:H26]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6