Detailed information of TA system TA137960

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4971899..4972739
• Replicon: chromosome
• Accession: NZ_CP043217
• Organism: Escherichia coli O80:H26 strain EC-107

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: FYA02_RS24660
• Protein ID: WP_000854686.1
• Coordinates: 4972356..4972739 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: FYA02_RS24655
• Protein ID: WP_001285602.1
• Coordinates: 4971899..4972279 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA02_RS24630	4969015..4969833	+	819	WP_001234732.1	DUF945 domain-containing protein	-
FYA02_RS24635	4969925..4970410	+	486	WP_000214307.1	antirestriction protein	-
FYA02_RS24640	4970426..4970902	+	477	WP_001186726.1	RadC family protein	-
FYA02_RS24645	4970965..4971186	+	222	WP_000692298.1	DUF987 domain-containing protein	-
FYA02_RS24650	4971205..4971888	+	684	WP_000086769.1	hypothetical protein	-
FYA02_RS24655	4971899..4972279	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FYA02_RS24660	4972356..4972739	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
FYA02_RS24665	4972736..4973224	+	489	WP_001054233.1	hypothetical protein	-
FYA02_RS24670	4973241..4973438	+	198	WP_000839253.1	DUF957 domain-containing protein	-
FYA02_RS24675	4973523..4974365	+	843	WP_001274547.1	DUF4942 domain-containing protein	-
FYA02_RS24685	4974836..4975774	+	939	WP_000351315.1	glyoxylate/hydroxypyruvate reductase GhrA	-
FYA02_RS24690	4975829..4976566	+	738	WP_000283664.1	zinc-binding phosphatase	-
FYA02_RS24695	4976590..4977144	+	555	WP_001001902.1	molecular chaperone YcdY	-
FYA02_RS24700	4977246..4977737	+	492	WP_001300785.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T137960 WP_000854686.1 NZ_CP043217:4972356-4972739 [Escherichia coli O80:H26]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T137960 NZ_CP043217:4972356-4972739 [Escherichia coli O80:H26]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT137960 WP_001285602.1 NZ_CP043217:4971899-4972279 [Escherichia coli O80:H26]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT137960 NZ_CP043217:4971899-4972279 [Escherichia coli O80:H26]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG