Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4280884..4281502 Replicon chromosome
Accession NZ_CP043217
Organism Escherichia coli O80:H26 strain EC-107

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FYA02_RS21230 Protein ID WP_001291435.1
Coordinates 4280884..4281102 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FYA02_RS21235 Protein ID WP_000344800.1
Coordinates 4281128..4281502 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA02_RS21195 4276173..4276745 + 573 WP_000779831.1 YbaY family lipoprotein -
FYA02_RS21200 4276776..4277087 - 312 WP_000409911.1 MGMT family protein -
FYA02_RS21210 4277466..4277819 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FYA02_RS21215 4277861..4279411 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FYA02_RS21220 4279575..4280045 - 471 WP_000136192.1 YlaC family protein -
FYA02_RS21225 4280161..4280712 - 552 WP_000102564.1 maltose O-acetyltransferase -
FYA02_RS21230 4280884..4281102 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FYA02_RS21235 4281128..4281502 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FYA02_RS21240 4282048..4285197 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FYA02_RS21245 4285220..4286413 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T137959 WP_001291435.1 NZ_CP043217:c4281102-4280884 [Escherichia coli O80:H26]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T137959 NZ_CP057210:c707799-707590 [Escherichia fergusonii]
ATGCTGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT137959 WP_000344800.1 NZ_CP043217:c4281502-4281128 [Escherichia coli O80:H26]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT137959 NZ_CP043217:c4281502-4281128 [Escherichia coli O80:H26]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References